原位杂交法检测非病毒性肝炎人群肝组织输血传播病毒

To study the existence of transfusion transmitted virus (TTV) in liver tissue taken from the people with no viral hepatitis and it's pathogenicity. The existence of TTV DNA in liver tissue was demonstrated by the method of in situ hybridization (ISH) . TTV DNA was detected in 9 from 32 cases (28.1%) by the method of in situ hybridization. The positive signals were localized in nucleus mainly or weakly expression in cytoplasm of liver cell. The positive cells were scatter or cluster form distributed in the lobule. Among 9 positive cases, 6 liver samples could been found mild histological changes (66.7%) , such as swollen, acidophilic degeneration, and lymphocytic infiltrate in portal tract, which were seminal to changes of hepatitis. Those histopathological changes could been seen only in 6 from 23 TTV DNA negative cases (26.1%) (x 2=4.48, P=0.048) . TT virus infection is more common in people with no hepatitis. Most of them had a mild histopathological changes although their serological tests could be kept normal.