TGF-β1和smad 4mRNA在慢性病毒性肝炎、肝硬化组织中的表达及意义

Hybridization in situ and im munohistochemistry were used in 10 cases of normal liver tissue and 70 cases of chronic viral hepatitis and cirrhosis.The results showed increased expression of TGF-β1 and smad4mRNA in the middle degree of chronic viral hepatitis and cirrh osis.the positive rates of smad4mRNA were obviously higher in the middle degree of chronic viral hepatitis and cirrhosis (87.5%, 82.6%) than in the normal liver (20%) , P<0.01;Meanwhile, TGF-β1 were strikingly higher in middle degree of chronic viral hepatitis and cirrhosis (83.33%, 86.96%) than in the normal liver (2 0%) , P<0.01 and the light degree of chronic viral hepatitis (52.2%) , P< 0.05 The most of both smad4mRNA and TGF-β1 positive cell were distributed of a round the enlarged portal aveas central vein, sinus The expression of TGF-β1 and smad4mRNA showed positive correlation in statistic.The relationship between exp ression of TGF-β1 and smad4mRNA reflected a state whether fibrosis formation wa s in activity or in regression.TGF-β1/smad4 pathway showed an important signifi cance in formation and development of fibrosis of chronic viral hepatitis.