Effects of knocking down IGFBPrP1 with siRNA on extracellular matrix secretion in HSC-T6

Objective To investigate the effects of inhibiting insulin-like growth factor binding protein related protein1 (IGFBPrP1) by chemically synthesized small interfering RNA (siRNA) on the secretion of extracellular matrix (ECM) in HSC-T6 and to determine the effect of IGFBPrP1 on the hepatic fibrosis.Methods (1) Two pairs of chemically synthesized siRNAs targeting IGFBPrP1 were respectively transfected into HSC-T6 cells for evaluation of silence efficacy and the one with higher efficacy was used in the following experiments; (2) Divide the HSC-T6 cells into three groups as follows: normal control group, negative control group and IGFBPrP1 siRNA group.HSC-T6 was treated by IGFBPrP1 siRNA, then the expressions of IGFBPrP1 and collagenⅠand fibronectin were detected by Western Blot.The data was analyzed by one-way ANOVA and SNK-q test.Results (1) Two pairs of siRNAs were transfected into HSC-T6.One pair of siRNA, which can highly effectively inhibit expression of IGFBPrP1 gene was screened successfully; (2) The expressions of IGFBPrP1 and collagenⅠand fibronectin in IGFBPrP1 siRNA group were significantly decreased than those in normal control group and negative control group (F﹦48.018, 81.246, 43.850, P﹤0.01) .Conclusion siRNA targeting IGFBPrP1 specifically inhibits the expression of IGFBPrP1 in HSC resulting in decreased synthesis and secretion of collagenⅠand fibronectin in hepatic stellate cells.So IGFBPrP1 can play an important role in liver fibrosis.