中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 3
Mar.  2012

The influence of Zuogui Wan Extract on bone marrow stem cell transforming liver cell

Research funding:

 

  • Published Date: 2012-03-20
  • Objective To observe the influence of Zuogui Wan extract on bone marrow stem cell transforming liver cell, and establish experimental basis to further study effective constituent of Zuogui Wan.Methods TranswellR insert was used to establish bone marrow stem cell-liver cell co-culture system.Zuogui Wan extract was prepared by water boiled-alcohol sink method, and was added to co-culture system.Bone marrow stem cell in TranswellR insert was detected after co-culture 7, 14, 21, 28 and 35 days.Glycogen was detected by PAS method, liver cell markers (AFP, Alb and CK18) were detected by immunocytochemistry method.Results Zuogui Wan extract influenced bone marrow stem cell survive when its concentration higher than 1.56mg/ml.The expression of glycogen, AFP, Alb and CK18 in bone marrow stem cell had no significant difference contrasted to control groups, when Zuogui Wan extract's concentration was 1.56mg/ml in co-culture system.Conclusion Bone marrow stem cell was sensitive to culture condition.Traditional Chinese medicine extract prepared by water boiled-alcohol sink method was unfit for experiment of bone marrow stem cell-liver cell co-culture system.

     

  • [1]李瀚旻, 高翔, 晏雪生, 等.左归丸促进骨髓形成肝细胞的研究[J].世界华人消化杂志, 2005, 13 (24) :2818-2822.
    [2]李瀚旻, 高翔, 晏雪生, 等.左归丸促进骨髓形成肝细胞的分子机制研究[J].中医杂志, 2006, 47 (10) :778-780.
    [3]李瀚旻.全面系统深入地研究中医药调控肝再生 (专论) [J].中西医结合肝病杂志, 2007, 17 (3) :129-132.
    [4]李瀚旻, 桂文甲, 李晶津, 等.左归丸对同种异性骨髓移植小鼠肝再生相关基因信号通路的影响[J].中国组织工程研究与临床康复, 2008, 12 (31) :6069-6073.
    [5]李瀚旻.中医再生医学概论[J].中华中医药学刊, 2008, 26 (11) :2309-2312.
    [6]李瀚旻.论“肝主生发”[J].中华中医药学刊, 2009, 27 (10) :2021-2025.
    [7]李瀚旻.肾藏精的科学内涵[J].中医杂志.2009, 50 (12) :1061-1064.
    [8]高翔, 李瀚旻, 晏雪生.左归丸对同种异性骨髓移植小鼠肝组织Wnt信号通路的影响[J].中西医结合肝病杂志, 2010, 20 (1) :29-31.
    [9]李瀚旻.肝藏象肝脏中心说[J].世界中医药杂志, 2011, 6 (1) :11-15.
    [10]李瀚旻.虚证本质与生机学说[J].中华中医药学刊, 2011, 29 (10) :2157-2160.
    [11]李瀚旻, 晏雪生, 罗建君, 等.左归丸药物血清对骨髓间质细胞转化为肝细胞的作用[J].中国组织工程研究与临床康复, 2007, 11 (28) :5465-5468.
    [12]李瀚旻, 高翔, 晏雪生.基于骨髓干细胞与肝组织细胞共培养体系的左归丸血清药理学研究[J].中国组织工程研究与临床康复, 2010, 14 (19) :3527-3532.
    [13]Koc ON, Gerson SL, Cooper BW, et al.Rapid hematopoiet-ic recovery after coinfusion of autologous blood stem cells and culture expanded marrow mesenchymal stem cells in ad-vanced breast cancer patients receiving high dose chemo-therapy[J].J Clin Oncol, 2000, 18 (2) :307-316.
    [14]Noort WA, Kruisselbrink AB, in't Anker PS, et al.Mesen-chymal stem cells promote engraftment of human umbilical cord blood-derived CD34 (+) cells in NOD/SCID mice[J].Exp Hematol, 2002, 30 (8) :870-878.
    [15]Abrahamsson CK, Yang F, Park H, et al.Chondrogenesis and mineralization during in vitro culture of human mesenchy-mal stem cells on three-dimensional woven scaffolds[J].Tissue Eng Part A, 2010, 16 (12) :3709-3718.
    [16]Gargett CE, Schwab KE, Zillwood RM, et al.Isolation and culture of epithelial progenitors and mesenchymal stem cells from human endometrium[J].Biol Reprod, 2009, 80 (6) :1136-1145.
    [17]Ninomiya Y, Sugahara-Yamashita Y, Nakachi Y, et al.De-velopment of a rapid culture method to induce adipocyte dif-ferentiation of human bone marrow-derived mesenchymal stem cells[J].Biochem Biophys Res Commun, 2010, 394 (2) :303-308.
  • Cited by

    Periodical cited type(0)

    Other cited types(1)

  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (3491) PDF downloads(646) Cited by(1)
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return