To probe the effect of TNF-α on express of matrix metalloproteinases (MMPs) and IL-8 in a culture medium of human hepatic stellate cells (hHSC) .hHSC were cultured and stimulated by TNF-α.MMPs and IL-8 in a culture were detected by ELISA at different time after TNF-α stimulation.The results shown that MMP-1, MMP-2 and IL-8 level was in turn at 28h>24h>20h>4h—16h (P<0.05-0.001) , MMP-3 at 28h>24h (P<0.01) >20h>4h—16h (P<0.05) .Average level of MMP-1 and MMP-2 in TNF-α group was higher than control after a 8 hours stimulation respectively (P<0.05) , and average level of MMP-3 was not significantly higher than control till at 20h (P<0.01) .The present data indicated that TNF-α could stimulate hHSC to express MMP-1, MMP-2, MMP-3 and IL-8, and hHSC not stimulated by TNF-α may express a little MMPs but no IL-8.
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