The effects of oxymatrine on apoptosis induced by TNF-α in human liver cell line L02

Wang JunXue; Wang GuoJun; Cai Xiong

Issue 4

Apr. 2001

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Article Navigation > Journal of Clinical Hepatology > 2001 > 17(4): 210-211+217

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氧化苦参碱对肿瘤坏死因子诱导的人L02肝细胞凋亡的影响

Published Date: 2001-04-20

Abstract

Abstract

we investigate the effects of Oxy on apoptosis induced by rhTNF α in human liver cell line L02.Cell viability and proliferation was assayed by trypan blue stain and H 3 TdR co operalion assay.Flowcytometer was used for the cell cycle analysis using propidium iodide DNA binding.Apoptosis can be induced by rhTNF α in L02 cells.Evaluation of the PI DNA binding by flow cytometry showed abolition of G1, S, and G2/M phases and their replacement by a sub 2N AP peak of fragmented DNA.Characteristic apoptic condensations, ranging from single ball condensations to budding, and apoptic bodies were found in cells.Cells treated with Oxy+rhTNF α 15ng/ml partly retain the ability of proliferation, showing no significantly sub 2N Ap peak of fragmented DNA by flow cytometry.Oxy may have inhibitory activity on apoptosis of L02 cells induced by rhTNF α.
- Oxymatrine,
- Human Tumor Necrosis Factor α,
- apoptosis

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[3]田晓华, 丛建波, 孙存普, 等氧化苦参碱清除·OH自由基及对胸腺嘧啶核苷酸辐射防护作用的ESR研究[J]解放军预防医学杂志, 1996, 14 (6) ∶412

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[7] 张琪氧化苦参碱对小鼠腹腔肥大细胞组织胺释放的抑制作用及其机制研究[J]中华微生物和免疫学杂志, 1992, (1) ∶41

[8] 徐伟明, 焦炳华, 曹雪涛等, 肿瘤坏死因子及其相关毒素[M ]上海科学技术出版社, 1991, p113

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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氧化苦参碱对肿瘤坏死因子诱导的人L02肝细胞凋亡的影响

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氧化苦参碱对肿瘤坏死因子诱导的人L02肝细胞凋亡的影响

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