CA/PEI亲和膜去除肝硬化腹水中内毒素及胆红素的研究

Objective To investigate the removal of endotoxin and bilirubin from ascites of patients with cirrhosis by cellulose diacetate /polyethyleneimine affinity membrane in vitro.Methods The CA/PEI affinity membrane was prepared by employing cellulos diacetate as matrix immobilized polyethyleneimine as affinity ligand, and the dynamic absorption of ascites through CA/PEI affinity membrane was conducted.The concentration of endotoxin, bilirubin, albumin, immunoglobulin (IgG, IgA and IgM) and electrolytes (K+, Na+ and Cl-) in the ascites before and after absorption were determined.Results The endotoxin and bilirubin level were greatly reduced (P﹤0.01) after absorption.There were no markedly changes for the concentration of albumine, immunoglobulin and electrolytes in the ascites.Conclusion CA/PEI affinity membrane can effectivly absorb the endotoxin and bilirubin from the ascites, and the absorption has no obviously influence on the albumine, immunoglobulin and electrolytes at the same time.