Recent thymic output function in patients with chronic hepatitis C by real time fluorescence quantitative PCR analysis

Guo HaiYan; Chen Ming; Wang LiPing; Ge GuoHong; Zhang YanChao

Issue 6

Jun. 2007

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Article Navigation > Journal of Clinical Hepatology > 2007 > 23(6): 432-433

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实时荧光定量PCR分析慢性丙型肝炎患者胸腺近期输出功能

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Published Date: 2007-06-20

Abstract

Abstract

Objective To analyze the content of T-cell receptor excision circles (TRECs) within peripheral blood mononuclear cells of chronic hepatitis C (CHC) patients to value the thymic output function of CHC and investigate the relation of thymic output function and HCV RNA in chronic hepatitis C (CHC) patients.Methods Quantitative detection of TREcs in DNA of peripheral blood mononuclear cells (PBMCs) from 22cases with CHC, 16 normal individuals was performed by real-time fluorescence quantitative PCR, and detection of HCV RNA of peripheral blood serum in CHC by RT-PCR with fluorescent probe technology.Results The mean of TRECs in patients with CHC (5.52±1.87 copies/103PBMCs) was lower than that in normal individuals (7.19±2.42 copies/103 PBMCs, P<0.05) ;The value of TRECs in patients with CHC was inversely correlated with HCV RNA (γ=-0.427, P<0.05) .Conclusion TRECs levels in CHC are lower in the healthy and inversely correlated with HCV RNA.
- chronic hepatitis C (CHC) ,
- TCR rearrangement excision circle (TRECs) ,
- recent thymic output function,
- real-time fluorescence quantitative PCR

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沈阳化工大学材料科学与工程学院沈阳 110142

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实时荧光定量PCR分析慢性丙型肝炎患者胸腺近期输出功能

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实时荧光定量PCR分析慢性丙型肝炎患者胸腺近期输出功能

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