Correlation between the recent thymic output function and the frequency of nave T cell in CHB patients

Li Li; Chen Ming; Pan XiuCheng; Wang LiPing; Fu JuanJuan; Feng Xia

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Feb. 2011

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Article Navigation > Journal of Clinical Hepatology > 2011 > 27(2): 157-160

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Correlation between the recent thymic output function and the frequency of nave T cell in CHB patients

Published Date: 2011-02-20

Abstract

Abstract

Objective To test the content of T cell receptor excision circles (TRECs) and the frequency of naive T lymphocytes in patients with chronic HBV infection and analyze their correlation.Methods Peripheral blood were collected from normal individuals and patients with chronic HBV infections. (1) TRECs were assessed by real-time PCR; (2) Lymphocytes were labeled with fluorescent-labeled antibodies FITC-CD3, PE-CD45RA and PE-cy5-CD62L and flow cytometry was used to detect the expressions of CD3, CD45RA and CD62L on the surface of T lymphocytes.Results (1) The TRECs (6.78±1.06) copies/103 in patients (n=25) with chronic HBV infections was significantly lower than that (8.16±2.07) copies/103 in healthy controls (n=25) (P<0.01) .The frequency of nave T cells (20.73±6.75) % in patients (n=25) with chronic HBV infections was significantly lower than that (27.82±9.66) % in healthy controls (n=25) (P<0.05) . (2) Both in patients with chronic HBV infections and in healthy controls, the frequency of nave T cells were in positive linear correlation with the TRECs, while the coefficient correlation were 0.688 (P<0.01) and 0.646 (P<0.01) respectively.The coefficient correlation in the two groups had no statistical differences, (P>0.05) .Conclusion Both the nave T cell in peripheral blood and the TRECs in patients with chronic HBV infection were decreased compare to the healthy adult, and the two indices had better linear correlation.
- hepatitis B,
- chronic,
- receptors,
- antigen

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[1]李红兵, 陈明, 汪莉萍, 等.实时荧光定量PCR测定胸腺近期输出功能方法的建立和应用[J].中华实验和临床病毒学杂志, 2007, 21 (1) :73-75.

[2]赵婷婷, 杨智清, 邹丽云, 等.慢性乙型病毒性肝炎患者外周血T淋巴细胞CD27和CD45RA表达的研究[J].免疫学杂志, 2006, 22 (6) :645-647.

[3]中华医学会.病毒性肝炎防治方案[J].中华肝脏病杂志, 2000, 8 (6) :324-329.

[4]Fernandez S, Nolan RC, Price P, et al.Thymic function in severely lmmunodeficient HIV type 1-infected patients receiving stable and effective antiretroviral therapy[J].AIDS Res Hum Retroviruses, 2006, 22 (2) :163-170.

[5]Zhang L, Lewin SR, Markowitz M, et al.Measuring recent thymic emigrants in blood of normal and HIV-1-infected individuals before and after effective therapy[J].J Exp Med, 1999, 190 (5) :725-732.

[6]Steffens CM, Smith KY, Landay A, et al.T cell receptor excision circle (TREC) content following maximum HIV suppression is equivalent in HIV-infected and HIV-uninfected individuals[J].AIDS, 2001, 15 (14) :1757-1764.

[7]Douek DC, McFarland RD, Keiser PH, et al.Changes in thymic function with age and during the treatment of HIV infection[J].Nature, 1998, 396 (6712) :690-695.

[8]葛国洪, 陈明, 李红兵, 等.正常人各年龄组外周血TRECs含量的检测[J].中华检验医学杂志, 2007, 30 (4) :463-465.

[9]Savage WJ, Bleesing JJ, Douek D, et al.Lymphocyte reconstitution following non-myeloablative hematopoietic stem cell transplantation follows two patterns depending on age and donor/recipient chimerism[J].Bone Marrow Transplant, 2001, 28 (5) :463-471.

[10]Cohen Stuart J, Hamann D, Borleffs J, et al.Reconstitu-tion of naive T cells during antiretroviral treatment of HIV-infected adults is dependent on age[J].AIDS, 2002, 16 (17) :2263-2266.

[11]Bas A, Hammarstrom SG, Hammarstrom ML.Extrathymic TCR gene rearrangement in human small intestine:Identification of new splice forms of recombination activating gene-1 mRNA with selective tissue expression[J].J Immunol, 2003, 171 (7) :3359-3371.

[12]Golden-Mason L, Douek DC, Koup RA, et al.Adult hu-man liver contains CD8pos T cells with naive phenotype, but is not a site for conventional alpha beta T Cell development[J].J Immunol, 2004, 172 (10) :5980-5985.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Correlation between the recent thymic output function and the frequency of nave T cell in CHB patients

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Correlation between the recent thymic output function and the frequency of nave T cell in CHB patients

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