Long-term culture and induce differentiation of hepatic stem cells from human fetal liver in vitro

Zhu ZhengYan; Du Zhi; Li Tao; Zhang JinJuan; Ma RuiLi

Issue 5

May 2004

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Article Navigation > Journal of Clinical Hepatology > 2004 > 20(5): 267-268

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人胎肝干细胞的体外培养及诱导分化

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Published Date: 2004-05-20

Abstract

Abstract

To separate hepatic stem cells (HSCs) from human fetal liver and to study their differentiation potentiality. Cells isolated from cord vein of fetal by perfusion digestion were cultured in vitro. Their characteristics were identified by immunocytochemistry. Cells isolated from fetal liver grew as colonies in vitro and could be subcultured stably. When the cells were cultured in the differential system, they could differentiated into hepatocytes. The results suggest the existence of bipotential hepatic stem cells in fetal liver, and their successful subculture paves the way for further study of bioartificial liver.
- fetal liver stem cell,
- cultured,
- differentiation

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人胎肝干细胞的体外培养及诱导分化

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人胎肝干细胞的体外培养及诱导分化

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