空气负离子对体外培养的肝癌SMMC-7721细胞的抑制作用及其相关的信号传导机制

Objective To investigate the affect on the biologic activity of hepatoma SMMC-7721 cell line of negative air ion in vitro.Methods Dividing the cell into three groups:negative control group, air control group and treatment group.Dividing the treatment group into 1h、2h、4h and 8h group according to action time of negative air ion, all the cells in each group were detected apoptosis and cell cycle with flow cytometry method, the phospho-P38MAPK expression was detected by Western blot assay.Results The apoptosis rate increased, the proportion of S stage cell decreased and the expression of phospho-P38MAPK increase obviously vs negative and air control group, the difference show significance (P<0.05) .Conclusion The negative air ion can induce hepatoma SMMC-7721 cell apoptosis through arresting the cell cycle, such effect was mediated by P38MAPK pathway.