The effect of inducing apoptosis and differentiation of all-trans retinoic acid (ATRA) on hepatocellular carcinoma (HCC) cells was studied with TRAP assay, Western blot assay and electron microscope. TRAP assay shows no ladder in ATRA group. While there are signs of expression of Caspase-3 and its subunit in the location of 32kD and 20kD seperetedly. It shows that ATRA can induce apoptosis. In Octreotide group, after 5 days of cultivation with Octreotide, there are condention of nuclear, reduction of microvillus, vacuolar degeneration of mitochondria, expression and activation of Caspase-3, and so on. Above all, the study suggests that ATRA and Octreotide have the effect of inducing indifferention and apoptosis seperatedly.
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