Study on Relationship between Detection of Hepatitis B Virus Replicative Intermediates RNA and Hepatitis B Virus DNA in Peripheral Blood Mononuclear Cells in Patients with Hepatitis B Virus Infection

Zhang KaiYu; Cai YanJun; Wang Feng; Xu Chang; Lu: MeiDe

Issue 3

Mar. 2001

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Article Navigation > Journal of Clinical Hepatology > 2001 > 17(3): 149-151

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乙肝患者外周血单个核细胞中乙肝病毒复制中间体RNA与乙肝病毒DNA相关性的研究

Published Date: 2001-03-20

Abstract

Abstract

We detected HBV replicative intermediates RNA in PBMCs in patients with HBV infection by RT PCR technology, meanwhile detected HBV DNA in PBMCs and serum by PCR technology.Five cases were detected for HBV RNA in PBMCs in 60cases of patients with HBV infection.HBV DNA were positive in both PBMCs in 26 cases and serum in 27 cases.In 26 cases whose HBV DNA in PBMCs were positive , 4 cases of HBV RNA in PBMCs were positive, In 27 cases whose HBV DNA in serum were positive, 3 cases of HBV RNA in PBMCs were positive.HBeAg was also detected in serum, in 17 cases whose HBeAg were positive, 4 cases of HBV RNA in PBMCs were positive.Though HBeAg and HBV DNA in serum, HBV DNA in PBMCs were negative in patients with HBV infection, HBV RNA in PBMCs may be positive, HBV in PBMCs could become the resource of recidimation and transmission.

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[1]DejeanA , lugassyC , ZafraniS , etal.DetectionofhepatitisBvirusDNAinpancreas , kidneyandskinoftwohumancarriersofvirus[J] .JournalofGeneralvirology , 1984, 6 5∶6 5 1.

[][2 ]Romet lemonneJ L , MclaneMF , ElfassiE , etal.HepatitisBvirusinfectioninculturedhumanlymphoblastoidcells[J] .Science , 1983 , 2 2 1∶6 6 7.

[3]DavisonF , AlexanderGJM , AnastassakosC , etal.Leucocytehepati tisBvirusDNAinacuteandchronichepatitisBvirusinfection[J] .JournalofMedicalvirology , 1987, 2 2∶379.

[4]黄育清 , 白敬羽 , 葛其童 , 等乙型病毒性肝炎患者外周血中单个核细胞及血清内HBVDNA的检测及其意义 [J] .临床检验杂志 , 1995 , 13 (1) ∶16

[][5 ]病毒性肝炎防治方案 (试行 ) [J] .中华传染病杂志 , 1995 , 13 (4) ∶2 41.

[][6 ]ChomczynskiPandSacchiN .Single stepmethodofRNAisolationbyacidguaridinumthiocyanate phenol chloroformextraction [J] .AnalBiochem , 1987, 16 2∶15 6 .

[7]SuT S , LuiW Y , LinH L , etal.AnalysisofhepatitisBvirustran scriptsininfectedhumanlivers[J] .hepatology , 1989, 19 (2 ) ∶180 .

[8]ShenHD , ChooKB , LeeSD , etal.HepatitisBvirusDNAinLeuko cytesofpatientswithhepatitisBvirus associatedliverdisease[J] .JMedVirol, 1986 , 18∶2 0 1.

[9]MillerRH , RobinsonWS .IntegratedhepatitisBvirusDNAse quencesspecifyingthemajorvirolcorepolypeptideandmethylatedinPLT/PRF/5cells[J] .ProcNatlAcadSciUSA , 1883 , 80∶2 5 34.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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乙肝患者外周血单个核细胞中乙肝病毒复制中间体RNA与乙肝病毒DNA相关性的研究

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乙肝患者外周血单个核细胞中乙肝病毒复制中间体RNA与乙肝病毒DNA相关性的研究

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