非酒精性脂肪性肝病是最常见慢性肝病的一种，本研究的两项相关实验旨在发现及确证其新的生物标志物。 首先：用蛋白质组学的方法分析70血清样本以寻找潜在的NAFLD生物标志物，其次：对6944名非NAFLD个体随访3年，以评估血红蛋白对NAFLD的预测价值。 第一个研究中，疾病组与对照组相比较，发现20个差异表达蛋白峰，运用生物信息学工具，我们建立了一个灵敏度为89%、特异度为83%的NAFLD生物标志物模型，进一步分析提示其中一个蛋白峰就是血红蛋白α亚单位。在第二项研究中，前瞻性分析表明高血红蛋白基线水平与NAFLD的高发生率相关，COX比例风险回归分析显示，调整年龄、性别、体重指数等变量下的风险比，基线血红蛋白水平在第2、3、4、5组的个体与第1组的相比较分别为1.36 1.66 1.76 1.83。本研究表明血清血红蛋白对于NAFLD可能具有很好的预测价值


Background & Aims：Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases worldwide. The two linked studies presented herein aimed to identify and verify new biomarkers for NAFLD.Methods：First, 70 serum samples were analyzed using proteomics approaches to identify potential biomarkers for NAFLD. Second, a total of 6944 initial NAFLD-free subjects were followed up for 3 years to evaluate the predictive value of hemoglobin for NAFLD. Results：In the first study, 20 differentially expressed protein peaks (11 up-regulated and nine down-regulated) were observed in NAFLD patients upon comparison to the controls. With the aid of bioinformatic tools, we established a biomarker pattern for NAFLD with a sensitivity of 89% and a specificity of 83%. Further analysis suggested a protein peak to be hemoglobin subunit alpha. In the second study, prospective analysis showed that subjects with higher baseline hemoglobin levels were associated with higher incidence of NAFLD. Cox proportional hazards regression analyses showed that the age, gender, and body mass index adjusted hazard ratio (95% CI) for subjects with baseline hemoglobin level in quintile 2, 3, 4, and 5 vs. quintile 1 was1.36 (1.02–1.81), 1.66 (1.23–2.25), 1.76 (1.28–2.41), and 1.83(1.33–2.53), respectively.Conclusions：Our study showed that serum hemoglobin may have significant predictive value for NAFLD.

点击下载 蛋白质组学分析提示血清血红蛋白对非酒精性脂肪性肝病有诊断价值