The relationship between of hematopoietic stem cells and hepatitis B virus infection

Objective To investigate the impact of HBV infection on the bone marrow haematopoietic stem cell (HSC) .Methods Nine hepatitis B patients and 7 healthy donors were included in this study.Bone marrow samples were collected and the CD34+ cells were purified by mini-MACS.HSCs were studied by immunohistochemiststy and hybridization in situ.HepG2.21 and HSC of healthy donors were used as a positive and negative control, respectively.HSCs were incubated with or without cytokine markers (SCF, FLT3, TPO, IL-3) .HBVDNA was detected by PCR and cells were counted on day 0, 1, 6 and 12 day.Results The immunohistochemistry result of HSCs in HBV patients showed blue-purple color among positive controls compared to healthy control group.The number of HSCs in the patient group was less than healthy control group on day 1, 6 and 12 after incubation with or without cytokines (t=0.818, P<0.05;t=3.599, P<0.05;t=2.967, P<0.05) .HBVDNA was higher after incubation with cytokine in the patient group than healthy control group on day 6 and 12 (t=3.36, P<0.05;t=5.71, P<0.01) .Conclusion HBV infects and replicate in bone marrow inhibiting the proliferation ability of HSCs.