Changes in serum level of interleukin-32 and interleukin-6 in patients with chronic hepatitis B virus infection and its clinical significance

Objective To explore the changes of interleukin-32 and interleukin-6 in serum of patients with hepatitis B virus infection and its clinical significance.Methods The serum IL-32 and IL-6 of 92 HBV-infected patients and 30 healthy individuals were detected by ELISA.Results ⑴There was statistical difference in serum IL-32 between the patient in hepatitis B group and control group (F=108.494, P<0.001) .The level of serum IL-32 in patient with AHB is the highest among the subjects.The level of IL-32 in serum was gradually increased in patients with mild, moderate and severe chronic hepatitis B.The level of serum IL-6 in hepatitis B group has significant difference compare with control group (F=139.256, P<0.001) , moreover the level of IL-6 in serum gradually increased in patients with mild, moderate and severe chronic hepatitis B.⑵The serum levels of IL-32, IL-6 in the HBV-infected patients with HBV DNA positive group were higher than those observed in the control group, however, no significant difference was observed between the groups.The differences among various HBV viral load groups were also not statistically significant (P>0.05) .⑶The serum level of IL-32 was positively correlated with IL-6, the correlation coefficient r was 0.70 (P<0.05) .Conclusion ⑴The results indicate that the level of IL-32, IL-6 increases in HBV-infected patients.The level of IL-32, IL-6 increases with the aggravation of inflammation of liver, indicating that IL-32, IL-6 play an important role in inflammatory response and are responsible for liver inflammatory injury process and diseases progression in HBV-infected patients.⑵It seems that the changes in serum IL-32 and IL-6 were not related to the HBV replication status.