骨髓干细胞在大鼠肝再生环境中的分化

To investigate the differentiation of rat bone marrow stem cells in liver regeneration.Bone marrow cells were obtained from the tibia of rat with partial hepatectomy.The bone marrow stem cells were enriched from the bone marrow cells by fluorescence-activated cell sorting.The bone marrow stem cells were labeled with PKH26-GL in vitro and then autotransplanted by portal vein injection.After 2 weeks, the livers were examined by albumin and ck 8 immunohistochemistry.The results show that there were PLH26-GL-labeling cells among hepatocytes in the liver sections, and some of the cells expressed albumin and ck 8.The results suggest that rat bone marrow stem cells can differentiate into hepatocyte in regenerative environment after partial hepatectomy, bone marrow stem cells may participate in liver regeneration after partial hepatectomy.