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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 2
Feb.  2011
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The protective effects of bone-marrow-derived mesenchymal stem cells on hepatic regeneration and repair of rat liver graft after reduced-size liver transplantation

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  • Published Date: 2011-02-20
  • Objective To explore the effect of bone-marrow-derived mesenchymal stem cells (BMSCs) in promoting regeneration and repair of rat liver after reduced-size liver transplantation.Methods (1) BMSCS which were taken from the bone marrow of the Wistar rats were cultured in vitro, then marked by CSFE and prepared as suspension. (2) 50% reduced-size liver transplantation models (SD donor to Wistar recipient) were established, which were divided into the experiment group (1ml BMSCs suspension was injected into portal vein) and the control group (1ml saline was injected into portal vein) .After operation, the living state of recipient was observed and the indicators about hepatic regeneration were detected through which serum and liver samples were collected and analyzed by biochemistry and immunohistochemistry respectively after transplantation.Results (1) BMSCs showed a high purity and homogeneity. (2) 2 hours after liver transplantation, all rats were recovered moving freely and drinking water. (3) There were BMSCs in the liver graft.The ratio between liver weight and body weight in the experimental group was higher than the control group.Histopathology revealed that hepatocyte injury was minor in the experimental group than the control group.Conclusion BMSCs transfused into the liver graft can promote both regeneration and repair of liver cells after reduced-size liver transplantation.

     

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  • [1]Pelersen BE, Bowen WC, Patrene KD, et al.Bone marrow as a potential of hepatic oval cells[J].Science, 1999, 284 (5417) :1168-1170.
    [2]Theise ND, Badve S, Saxena R, et al.Derivation of hepatocytes from bone marowcells in mice after radiation induced myeloahlation[J].Hepatology, 2000, 3l (1) :235-240.
    [3]Polehert D, Sobinsky J, Douglas G, et al.IFN-gamma activation of mesenchymal stem cells for treatment and prevention of graft versus host disease[J].Eur J Immunol, 2008, 38 (6) :1745-1755.
    [4]Oh SH, Miyazaki M, Kouchi H, et al.Hepatocyte growthfactor induce differentiation of adult rat bone marrow cells intoa heoatocyte lineage in vitro[J].Biochem Biophys Res Commun, 2000, 279 (9) :500-504.
    [5]Schwartz RE, Reyes M, Koodiel L, et al.Multipotent adult progenitor cells from bone marrow differentiate into functional hepatic like cells[J].J Clin Invest, 2002, 109 (10) :1291-1302.
    [6]周播江, 钟翠平, 顾云娣, 等.肝再生大鼠血清诱导骨髓干细胞向肝细胞分化的实验研究[J].中华肝脏病杂志, 2004, l2 (12) :730-733.
    [7]Kim HS, Lee HJ, Yeu IS, et al.The neovascularization effect of bone marrow stromal cells in temporal muscle after encephalomyosynangiosis in chronic cerebral ischemic rats[J].J Korean Neurosurg Soc, 2008, 44 (4) :249-255.
    [8]Sell S.Heterogeneity and plasticity of hepatocyte lineage cells[J].Hepatology, 2001, 33 (3) :738-750.
    [9]Oostendorp RA, Audet J, Eaves CJ, et al.High resolution tracking of cell division suggests similarcell cycle kinetics of hematopoietic stem cells stimulatedin vitroandin vivo[J].Blood, 2000, 95 (3) :855-862.
    [10]邵永, 董家鸿, 王曙光, 等.近交系大鼠肝移植急性排斥模型的建立及排斥反应观察[J].徐州医学院学报, 2008, 28 (9) :561-564.
    [11]Kitada M, Dezawa M.Induction system of neural and muscle lineage cells from bone marrow stromal cells;a new strategy for tissue reconstruction in degenerative diseases[J].Histol Histopathol, 2009, 24 (5) :631-642.
    [12]Li ZJ, Teng BX, Chen XH, et al.Leukemic bone marrow stromal cells in vitro protect leukemic cell line Jurkat cells from daunorubicin-induced apoptosis[J].Ai Zheng, 2005, 24 (6) :672-675.
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