中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 1
Jan.  2011

Packaging, selection and identification of recombinant adenoviruses carrying HCV HLA-A2 restricted compound multi-epitopes antigen

Research funding:

 

  • Published Date: 2011-01-20
  • Objective To construct recombinant shuttle plasmid pAdtrack-CMV-Ub-Mep carrying HCV HLA-A2 restricted compound multi-epitopes gene, and transfect to HEK293 cells, to obtain recombinant adenoviruses rAd-Ub-Mep.Methods The compound antigen gene Ub-Mep was cloned into shuttle plasmid pAdTrack-CMV for pAdtrack-CMV-Ub-Mep.The recombinant adenoviruses rAd-Ub-Mep was packed in HEK293 cells by co-transfection with pAdtrack-CMV-Ub-Mep and frame plasmid pAdEasy1.The recombinant adenoviruses rAd-Ub-Mep was identified by PCR and expression of GFP, and the virus titer was determined by TCID50.Results The recombinant adenoviruses rAd-Ub-Mep was confirmed by enzyme cutting, PCR and fluorometric analysis.The virus titer was 1.2×1011cfu/L.Conclusion Success to construct recombinant adenoviruses vaccine, is the base for the study of cellular immunity induced by HCV HLA-A2 restricted compound multi-epitopes gene.

     

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