STAT3基因在原发性肝癌组织中的表达及意义

Objective To investigate the important role and significance of STAT3 gene during the progress of originating and developing of human hepatocellular carcinoma (HCC) .Methods Applying Reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the mRNA expression of STAT3, c-myc, p53, survivin and VEGF genes, the protein expression of STAT3 gene were detected by western blot and immunohistochemical method.Results The mRNA expression of STAT3 gene is significant higher in HCC and tissue beside carcinoma than in normal tissue (P<0.05) , and the mRNA expression of c-myc, survivin, VEGF genes were up-regalation in HCC and tissue beside carcinoma than in normal tissue (P<0.05) , the mRNA expression of p53 was down-regulation in HCC and tissue beside carcinoma than in normal tissue (P<0.05) , Overexpression of STAT3 protein was detected in HCC and tissue beside carcinoma than in normal tissue (P<0.05) .Conclusion The persistent activation of STAT3 gene plays an important role in the carcinogenesis of HCC, and STAT3 gene may be act as a new target in the diagnosis and therapy of HCC.