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缺氧诱导因子1α对肝癌细胞HepG2干细胞特性及表阿霉素敏感性的影响

赵金金 张海光 崔非非 汪磊 莫清江 焦路阳

赵金金, 张海光, 崔非非, 等. 缺氧诱导因子1α对肝癌细胞HepG2干细胞特性及表阿霉素敏感性的影响[J]. 临床肝胆病杂志, 2021, 37(2): 354-357. DOI: 10.3969/j.issn.1001-5256.2021.02.021
引用本文: 赵金金, 张海光, 崔非非, 等. 缺氧诱导因子1α对肝癌细胞HepG2干细胞特性及表阿霉素敏感性的影响[J]. 临床肝胆病杂志, 2021, 37(2): 354-357. DOI: 10.3969/j.issn.1001-5256.2021.02.021
ZHAO JJ, ZHANG HG, CUI FF, et al. Effect of hypoxia-inducible factor-1α on stemness and epirubicin sensitivity of HepG2 hepatoma cells[J]. J Clin Hepatol, 2021, 37(2): 354-357. DOI: 10.3969/j.issn.1001-5256.2021.02.021
Citation: ZHAO JJ, ZHANG HG, CUI FF, et al. Effect of hypoxia-inducible factor-1α on stemness and epirubicin sensitivity of HepG2 hepatoma cells[J]. J Clin Hepatol, 2021, 37(2): 354-357. DOI: 10.3969/j.issn.1001-5256.2021.02.021

缺氧诱导因子1α对肝癌细胞HepG2干细胞特性及表阿霉素敏感性的影响

DOI: 10.3969/j.issn.1001-5256.2021.02.021
基金项目: 

新乡医学院第一附属医院博士科研项目启动基金 10243

河南省医学科技攻关计划(联合共建)项目 LHGJ20190453

详细信息
    作者简介:

    赵金金(1987—),男,主管技师,博士,主要从事肿瘤耐药及肿瘤干细胞的研究

    通讯作者:

    焦路阳,jiaoluyang2009@163.com

  • 利益冲突声明:本研究不存在研究者、伦理委员会、受试者监护人以及与公开研究成果有关的利益冲突,特此声明。
  • 作者贡献声明:赵金金负责课题设计,资料分析,撰写论文;张海光负责实验操作,参与数据分析;崔非非、汪磊参与实验操作;莫清江参与收集数据,修改论文;焦路阳负责拟定写作思路,指导撰写论文并最后定稿。
  • 中图分类号: R735.7

Effect of hypoxia-inducible factor-1α on stemness and epirubicin sensitivity of HepG2 hepatoma cells

  • 摘要:   目的  探讨缺氧诱导因子(HIF)1α对肝癌细胞HepG2干细胞特性及表阿霉素敏感性的影响。  方法  以肝癌细胞为研究对象,肝癌细胞HepG2脂质体转染过表达HIF-1α的质粒作为实验组,转染pcDNA3.1空质粒作为对照组,单独HepG2细胞为HepG2组。实时荧光定量PCR检测HIF-1α mRNA表达,Western Blot检测HIF-1α蛋白表达;流式细胞术检测细胞表面CD133表达。不同浓度表阿霉素(0、6.25、12.5、25、50 μmol/L)作用3组细胞24 h,MTT法检测细胞活性,流式细胞术检测表阿霉素(50 μmol/L)处理后细胞凋亡情况。计量资料多组间比较采用单因素方差分析,进一步两两比较采用t检验。  结果  相较于HepG2组及对照组,实验组HIF-1α mRNA的表达水平明显升高,差异具有统计学意义(P值均<0.001);Western Blot结果显示实验组HIF-1α蛋白高表达。HepG2组、对照组和实验组细胞的CD133比例分别为0.040%±0.003%、0.030%±0.010%、20.110%±0.600%,实验组的CD133阳性率显著高于HepG2组和对照组(P值均<0.001)。表阿霉素浓度为25、50 μmol/L时,HepG2组和对照组的细胞活性明显受到抑制,显著低于实验组(P值均<0.05)。50 μmol/L表阿霉素作用48 h后,实验组的细胞凋亡率(67.9%±2.5%)较HepG2组(93.6%±1.5%)和对照组(93.0%±1.2%)明显降低(P值均<0.001)。  结论  过表达HIF-1α的质粒成功转染至HepG2细胞,HIF-1α可提高肝癌细胞干细胞比例使其对表阿霉素耐药。
  • 图  1  HIF-1α质粒在HepG2细胞的表达

    注:a,real-time qPCR检测HIF-1α质粒在HepG2细胞的表达;b,Western Blot检测HIF-1α质粒在HepG2细胞的表达。

    图  2  流式细胞术检测HepG2细胞CD133表达

    图  3  流式细胞术检测细胞凋亡情况

    表  1  不同浓度表阿霉素作用24 h的细胞活性

    组别 表阿霉素浓度(μmol/L)
    0 6.25 12.5 25 50
    HepG2组(%) 100 96.33±1.21 85.47±2.28 67.10±3.56 42.27±0.67
    对照组(%) 100 92.60±1.54 86.80±2.40 59.03±2.60 45.50±0.56
    实验组(%) 100 92.50±0.35 85.53±0.81 84.10±1.181)2) 72.30±2.071)2)
    F 3.618 0.696 23.570 162.200
    P 0.093 0.535 0.001 <0.001
    注:与对照组比较,1)P<0.05;与HepG2组比较,2)P<0.05。
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出版历程
  • 收稿日期:  2020-08-12
  • 修回日期:  2020-10-10
  • 刊出日期:  2021-02-20
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