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内质网应激对肝泡型包虫病部分炎症介质的影响

李姚 王靖超 袁加琪 温浩 周瀛 樊海宁 王志鑫

李姚, 王靖超, 袁加琪, 等. 内质网应激对肝泡型包虫病部分炎症介质的影响[J]. 临床肝胆病杂志, 2021, 37(1): 131-134. DOI: 10.3969/j.issn.1001-5256.2021.01.026
引用本文: 李姚, 王靖超, 袁加琪, 等. 内质网应激对肝泡型包虫病部分炎症介质的影响[J]. 临床肝胆病杂志, 2021, 37(1): 131-134. DOI: 10.3969/j.issn.1001-5256.2021.01.026
LI Y, WANG JC, YUAN JQ, et al. Influence of endoplasmic reticulum stress on the secretion of some inflammatory mediators in hepatic alveolar echinococcosis [J]. J Clin Hepatol, 2021, 37(1): 131-134. DOI: 10.3969/j.issn.1001-5256.2021.01.026
Citation: LI Y, WANG JC, YUAN JQ, et al. Influence of endoplasmic reticulum stress on the secretion of some inflammatory mediators in hepatic alveolar echinococcosis [J]. J Clin Hepatol, 2021, 37(1): 131-134. DOI: 10.3969/j.issn.1001-5256.2021.01.026

内质网应激对肝泡型包虫病部分炎症介质的影响

DOI: 10.3969/j.issn.1001-5256.2021.01.026
基金项目: 

青海省重大科技专项子课题 2016-SF-A5

2017年青海“高端创新人才千人计划” 培养拔尖人才项目

详细信息
    作者简介:

    李姚(1992—),男,主要从事肝胆胰外科、肝包虫病学研究

    王靖超(1994—),男,主要从事肝胆胰外科、肝包虫病学研究

    通讯作者:

    王志鑫,wangzhixin126@163.com

  • 利益冲突声明:本研究不存在研究者、伦理委员会成员、受试者监护人以及与公开研究成果有关的利益冲突,特此声明。
  • 作者贡献声明:李姚、王靖超、袁加琪、温浩、周瀛、樊海宁、王志鑫负责课题设计、资料分析; 李姚、王靖超负责收集数据与撰写论文; 李姚、王靖超、王志鑫参与修改论文; 温浩、周瀛、樊海宁、王志鑫责拟定写作思路,指导撰写论文并最后定稿。
  • 二人对本文贡献相同,同为第一作者
  • 中图分类号: R575;R535

Influence of endoplasmic reticulum stress on the secretion of some inflammatory mediators in hepatic alveolar echinococcosis

  • 摘要:   目的  探究内质网应激(ERS)在肝泡型包虫病(HAE)病情发展过程中对部分炎症介质的影响,初步探讨其临床意义。  方法  选取2018年6月—2019年9月因HAE在青海大学附属医院接受肝脏部分切除术的患者15例,切除HAE病灶边缘带作为AE组,同期肝脏正常组织15例作为对照组。通过蛋白质印迹法、实时荧光定量PCR检测AE组和对照组中的蛋白激酶R样激酶(PERK)、CCAAT/增强子结合蛋白同源蛋白(CHOP)、含半胱氨酸的天冬氨酸蛋白水解酶12(caspase-12)和葡萄糖调节蛋白78(GRP-78)表达水平以及IL-1β、IL-6和TNF的mRNA表达水平。两组间不满足正态性的计量资料比较采用Mann-Whitney U检验; 满足正态性的计量资料两组间分析采用t检验; 双变量相关性分析采用Pearson相关分析法。  结果  AE组中PERK、CHOP、caspase-12、GRP-78的蛋白表达量(U值分别为4.165、3.461、2.577、3.344,P值均<0.001)和mRNA表达水平(t值分别为34.003、4.461、53.573、55.224,P值均<0.001)均明显高于对照组,差异均有统计学意义。AE组中IL-1β、IL-6和TNF的mRNA表达水平明显高于对照组(t值分别为6.090、12.578、53.573,P值均<0.001)。PERK、CHOP、caspase-12、GRP-78的蛋白表达水平与IL-1β、IL-6和TNF的mRNA表达水平均呈正相关(r值均>0.700,P值均<0.05)。  结论  HAE中ERS的激活与炎症介质的活化存在正相关性,ERS的过度激活能够改变部分炎症介质的分泌来加剧肝脏的损伤,具体机制有待进一步研究。
  • 表  1  PCR引物序列

    基因 引物序列(5′-3′) 引物长度(bp)
    IL-1β R:CGATCACTGAACTGCACGCTC 168
    F:ACAAAGGACATGGAGAACACCACTT
    IL-6 R:CAATGAGGAGACTTGCCTGGTG 203
    F:TGGCATTTGTGGTTGGGTCA
    下载: 导出CSV

    表  2  两组间ERS相关蛋白表达水平的比较

    组别 例数 PERK CHOP caspase-12 GRP-78
    AE组 15 27.63(25.32~29.32) 37.36(35.34~39.52) 31.41(29.34~34.63) 30.89(28.34~33.12)
    对照组 15 19.89(18.34~21.28) 28.63(24.52~32.14) 20.36(17.62~24.83) 20.45(16.34~23.66)
    U 4.165 3.461 2.577 3.344
    P <0.001 <0.001 <0.001 <0.001
    下载: 导出CSV

    表  3  两组间ERS相关指标mRNA表达水平的比较

    组别 例数 PERK CHOP caspase-12 GRP-78
    AE组 15 27.445±0.553 37.007±1.364 31.252±0.568 30.927±0.536
    对照组 15 19.892±0.658 27.752±2.539 20.227±0.559 20.110±0.138
    t 34.003 4.461 53.573 55.224
    P <0.001 <0.001 <0.001 <0.001
    下载: 导出CSV

    表  4  两组间相关炎症介质mRNA表达水平的比较

    组别 例数 IL-1β IL-6 TNF
    AE组 15 3.265±1.215 33.461±5.991 26.368±2.472
    对照组 15 1.294±0.311 12.548±2.361 10.563±0.618
    t 6.090 12.578 53.573
    P <0.001 <0.001 <0.001
    下载: 导出CSV

    表  5  ERS相关蛋白与炎症介质的相关性

    指标 PERK CHOP caspase-12 GRP-78
    r P r P r P r P
    IL-1β 0.811 0.016 0.782 0.008 0.778 <0.001 0.786 0.005
    IL-6 0.922 0.020 0.879 0.032 0.934 0.012 0.917 <0.001
    TNF 0.784 <0.001 0.943 0.011 0.935 0.006 0.885 0.031
    下载: 导出CSV
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  • 收稿日期:  2020-05-20
  • 修回日期:  2020-09-09
  • 刊出日期:  2021-01-18
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