血管紧张素1-7对雨蛙素诱导的胰腺腺泡细胞Toll样受体4/核因子-κB通路的影响

王艳; 崔立建; 肖红丽; 王国兴; 阴赪宏

doi:10.3969/j.issn.1001-5256.2016.11.029

血管紧张素1-7对雨蛙素诱导的胰腺腺泡细胞Toll样受体4/核因子-κB通路的影响

DOI: 10.3969/j.issn.1001-5256.2016.11.029

1. 首都医科大学附属北京友谊医院急诊科
2. 首都医科大学附属北京妇产医院内科

基金项目:

国家自然科学基金资助项目(81441060)；首都医科大学附属北京友谊医院科研启动基金(yyqdkt2014-4)；

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中图分类号: R576
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出版历程
- 出版日期: 2016-11-20

Effect of angiotensin 1-7 on the TLR4/NF-κB pathway in cerulein-induced pancreatic acinar cells

Research funding:

摘要

摘要: 目的探讨血管紧张素1-7[Ang（1-7）]对雨蛙素诱导的胰腺腺泡细胞AR42J中Toll样受体（TLR）4/核因子（NF）-κB通路的影响。方法 AR42J细胞随机分为4组:对照组、模型组（10 nmol/L雨蛙素刺激）、Ang（1-7）组（10-7mol/L、10-6mol/L、10-5mol/L Ang（1-7）+10 nmol/L雨蛙素）及Ang（1-7）受体抑制剂A779组（10-7mol/L、10-6mol/L、10-5mol/L A779+10 nmol/L雨蛙素）。对照组为正常生长的AR42J细胞,模型组用10 nmol/L的雨蛙素刺激AR42J分别于15 min、30 min、2 h、6 h、12 h及24 h收集细胞,Ang（1-7）组为不同浓度Ang（1-7）作用30 min后再用雨蛙素刺激12 h收集细胞,A779组为不同浓度A779作用30 min后再用雨蛙素刺激12 h收集细胞。免疫荧光技术检测TLR4及NF-κB在AR42J中的表达部位,Western Blot技术检测各组细胞中TLR4及NF-κB的蛋白表达水平。计量资料组间比较采用单因素方差分析,进一步...
- 胰腺炎 /
- 细胞系 /
- 血管紧张素类 /
- Toll样受体4 /
- NF-κB
Abstract: Objective To investigate the effect of angiotensin 1- 7 [Ang( 1- 7) ] on the TLR4 / NF- κB pathway in cerulein- stimulated pancreatic acinar AR42 J cells. Methods AR42 J cells were randomly divided into control group,model group( stimulated with 10 nmol / L cerulein),Ang( 1- 7) group( 10- 7,10- 6,and 10- 5mol / L Ang( 1- 7) + 10 nmol / L cerulein),and Ang( 1- 7) receptor inhibitor A779group( 10- 7,10- 6,and 10- 5mol / L A779 + 10 nmol / L cerulein). The control group was AR42 J cells with normal growth. The AR42 J cells in the model group were stimulated with 10 nmol / L cerulein and the cells were collected at 15 minutes,30 minutes,2 hours,6 hours,12 hours,and 24 hours after stimulation. The AR42 J cells in the Ang( 1- 7) group were treated with various concentrations of Ang( 1- 7) for30 minutes and then stimulated with cerulein for 12 hours,and then the cells were collected. The AR42 J cells in the A779 group were treated with various concentrations of A779 for 30 minutes and then stimulated with cerulein for 12 hours,and then the cells were collected. Immunofluorescence assay was used to determine the expression sites of TLR4 and NF- κB in AR42 J cells,and Western blot was used to measure the protein expression of TLR4 and NF- κB in AR42 J cells in each group. A one- way analysis of variance was used for comparison of continuous data between groups,and the least significant difference method was used for further comparison between any two groups. Results Both TLR4 and NF- κB were expressed in the cytoplasm of AR42 J cells. Compared with the control group,the model group showed significant increases in the expression of TLR4 at 30 minutes,2 hours,and 6 hours after modeling and a significant reduction at 12 hours after modeling( all P < 0. 05),as well as significant increases in the expression of NF- κB at 30 minutes,2 hours,6 hours,12 hours,and 24 hours after modeling( all P < 0. 05). The 10- 5mol / L Ang( 1- 7) group had significantly downregulated protein expression of TLR4 and NF- κB compared with the model group( 0. 570 ± 0. 046 vs 0. 893 ± 0. 057,0. 520 ± 0. 071 vs 0. 750 ± 0. 057,both P < 0. 05).The 10- 5mol / L A779 group had significantly upregulated protein expression of TLR4 and NF- κB compared with the model group( 0. 680± 0. 045 vs 0. 563 ± 0. 088,0. 617 ± 0. 071 vs 0. 453 ± 0. 054,both P < 0. 05). Conclusion In cerulein- stimulated AR42 J cells,Ang( 1- 7) can downregulate TLR4 and inhibit NF- κB activation.
- pancreatitis /
- cell line /
- angiotensins /
- toll-like receptors 4 /
- NF-kappa B

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参考文献(18)

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