不同途径移植骨髓间充质干细胞治疗肝硬化小鼠的效果比较

张丽霞; 李莹; 王黎明; 严祥

doi:10.3969/j.issn.1001-5256.2016.10.016

不同途径移植骨髓间充质干细胞治疗肝硬化小鼠的效果比较

DOI: 10.3969/j.issn.1001-5256.2016.10.016

1. 兰州大学第一医院干二科
2. 兰州大学第一医院普外二科再生医学重点转化实验室

基金项目:

甘肃省重大专项项目(1302FKDA029)；

详细信息

中图分类号: R575.2
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出版历程
- 出版日期: 2016-10-20

Bone marrow mesenchymal stem cell transplantation via different approaches in treatment of liver cirrhosis in mice

Second Department of Cardre, The First Hospital of Lanzhou University

Research funding:

摘要

摘要:
目的评价经不同途径移植骨髓间充质干细胞(BMSC)治疗小鼠肝硬化的效果差异。方法 46只BALB/c小鼠随机分为正常对照组5只和肝硬化模型组41只。用CCl4橄榄油皮下注射构建小鼠肝硬化模型,选取肝硬化小鼠36只,随机分为对照组、尾静脉移植组、脾内移植组,每组各12只。以全骨髓贴壁法获得第3代BMSC,通过流式细胞分析术行细胞表面鉴定,选取尾静脉及脾脏注射至肝硬化小鼠体内。移植后4周分别取血检测肝功能,取肝组织切片进行HE、Masson染色及α平滑肌肌动蛋白(αSMA)免疫组化检查,检测小鼠肝细胞组织损伤和纤维化情况。组间比较采用单因素方差分析。结果造模8周后,模型组小鼠毛发变稀,色泽暗黄,摄食减少,活动减少,体质量下降。细胞移植后,脾内移植和尾静脉移植组小鼠Alb较对照组明显升高[(29.05±3.41)g/L vs(21.03±2.18)g/L,(29.46±3.25)g/L vs(21.03±2.18)g/L],ALT、AST明显降低[(170.67±32.72)U/L vs(254.58±47.93)U/L,(166.58±28.24)U/L vs(254.58±41.93)U...
- 肝硬化 /
- 间质干细胞移植 /
- 小鼠,近交BALB C
Abstract:
Objective To investigate the clinical effects of bone marrow mesenchymal stem cell( BMSC) transplantation via different approaches in the treatment of liver cirrhosis in mice. Methods A total of 46 BALB / c mice were randomly divided into normal control group with 5 mice and liver cirrhosis model group with 41 mice. Subcutaneously injected carbon tetrachloride olive oil was used to establish the mouse model of liver cirrhosis. A total of 36 mice with liver cirrhosis were randomly divided into control group,caudal vein BMSC transplantation group,and spleen BMSC transplantation group,with 12 mice in each group. Whole bone marrow adherent culture was performed to obtain the third-generation BMSCs,and flow cytometry was used for cell surface identification. BMSCs were injected into the mice through the caudal vein or spleen. Blood samples were collected at 4 weeks after transplantation to measure liver function. HE and Masson staining and α-smooth muscle actin( α-SMA) immunohistochemistry were performed for liver sections. Liver injury and fibrosis in mice were examined. A one-way analysis of variance was used for comparison between groups. Results At 8 weeks after the establishment of the model,the mice in the model group had sparse and dark yellow hair,reduced food consumption and activity,and a reduction in body weight.After transplantation,compared with the model control group,the caudal vein BMSC transplantation group and spleen BMSC transplantation group showed a significant increase in albumin and significant reductions in alanine aminotransferase and aspartate aminotransferase( all P<0. .="" there="" were="" no="" significant="" differences="" between="" the="" two="" transplantation="" p="">0. 05). After transplantation,there were significant changes in diseased tissue,alleviated liver cirrhosis,reduced collagen fiber and necrotic area,and a good structure. Immunohistochemistry showed both transplantation groups showed significant reductions in the number of cells with positive α-SMA. Conclusion BMSC transplantation via the tail vein or spleen can improve liver function and diseased tissue in mice with liver cirrhosis and these two approaches have comparable clinical effects.
- liver cirrhosis /
- mesenchymal /
- stem cells

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参考文献(18)

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