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基于GC-TOFMS和UPLC-QTOFMS代谢组学分析平台对轻微型肝性脑病特异性代谢物谱的分析

黄国初 王萌 杨小徽 姚凡 钟宇 姚春

黄国初, 王萌, 杨小徽, 姚凡, 钟宇, 姚春. 基于GC-TOFMS和UPLC-QTOFMS代谢组学分析平台对轻微型肝性脑病特异性代谢物谱的分析[J]. 临床肝胆病杂志, 2016, 32(1): 139-142. DOI: 10.3969/j.issn.1001-5256.2016.01.027
引用本文: 黄国初, 王萌, 杨小徽, 姚凡, 钟宇, 姚春. 基于GC-TOFMS和UPLC-QTOFMS代谢组学分析平台对轻微型肝性脑病特异性代谢物谱的分析[J]. 临床肝胆病杂志, 2016, 32(1): 139-142. DOI: 10.3969/j.issn.1001-5256.2016.01.027
Huang GuoChu, Wang Meng, Yang XiaoHui, Yao Fan, Zhong Yu, Yao Chun. Analysis of specific metabolite profiling of minimal hepatic encephalopathy based on metabolomics analytical platforms of GC-TOFMS and UPLC-QTOFMS[J]. J Clin Hepatol, 2016, 32(1): 139-142. DOI: 10.3969/j.issn.1001-5256.2016.01.027
Citation: Huang GuoChu, Wang Meng, Yang XiaoHui, Yao Fan, Zhong Yu, Yao Chun. Analysis of specific metabolite profiling of minimal hepatic encephalopathy based on metabolomics analytical platforms of GC-TOFMS and UPLC-QTOFMS[J]. J Clin Hepatol, 2016, 32(1): 139-142. DOI: 10.3969/j.issn.1001-5256.2016.01.027

基于GC-TOFMS和UPLC-QTOFMS代谢组学分析平台对轻微型肝性脑病特异性代谢物谱的分析

DOI: 10.3969/j.issn.1001-5256.2016.01.027
详细信息
  • 中图分类号: R575.3

Analysis of specific metabolite profiling of minimal hepatic encephalopathy based on metabolomics analytical platforms of GC-TOFMS and UPLC-QTOFMS

  • 摘要:

    目的分析对比健康志愿者及轻微型肝性脑病(MHE)患者的血清代谢物谱,确立MHE特异性生物标志物,探索MHE早期诊断方法。方法收集2013年10月-2014年10月广西中医药大学第一附属医院收治的乙型肝炎肝硬化导致的MHE患者29例(MHE组)和同期本院健康体检者50例(NC组),运用气相色谱-飞行时间质谱仪(GC-TOFMS)和超高压液相色谱-四级杆-飞行时间质谱仪(UPLC-QTOFMS)检测受试者血清样本中的代谢物,并通过主成分分析(PCA)、正交偏最小二乘法-判别分析(OPLS-DA)进行多维统计分析,结合单维t检验进行组间比较,最终确定与MHE相关的生物标志物。结果对2组进行了PCA和OPLS-DA建模分析,结合单维t检验结果,并参照模型中的VIP值,在2组血清中找到27种可能的MHE特异性生物标志物。根据OPLS-DA模型中MHE组和NC组代谢物质的比值可见,与健康志愿者相比,在MHE患者血清中下调的物质有9种,上调的物质有18种。结论基于GC-TOFMS和UPLC-QTOFMS平台的血清代谢组学分析技术可同时检测患者血清中多种代谢物质,有助于研究MHE的发病机制。本研究建立...

     

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  • 收稿日期:  2015-05-28
  • 刊出日期:  2016-01-20
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