Objective To evaluate the efficacy and safety of Baquting (hemocoagulase for injection) for hemostasis in biliary surgery.Methods A total of 240 patients were divided into hemocoagulase group (n = 120) and control group (n = 120) .Normal saline was used instead of hemocoagulase in the control group.The two groups were compared in terms of intraoperative blood loss, blood loss per unit area, total drainage volume at 3 d after operation, and transfusion volume of suspended red blood cells (RBCs) in the perioperative period, and the effect of hemocoagulase on coagulation function was evaluated.Comparison of measurement data was made by t test.Results Compared with the control group, the hemocoagulase group had significantly less intraoperative blood loss (94.3 ± 11.4 vs 128.6 ± 10.3 ml, t = 24.456, P < 0.05) , significantly less blood loss per unit area (0.2 ± 0.1 vs 0.3 ± 0.2 g / cm2, t =4.899, P<0.05) , and significantly lower transfusion volume of RBCs (0.7 ± 0.3 vs 1.9 ± 1.1 U, t = 11.529, P < 0.05) .There were no significant differences in coagulation function between the two groups before and after operation (P >0.05) .Conclusion Hemocoagulase is effective and safe in preventing and treating bleeding during and after biliary surgery and can reduce intraoperative and postoperative blood loss and requirement for suspended RBC transfusion in the perioperative period.