蛋白质组学及代谢组学在酒精性肝病中的应用

王晓玉; 罗建平; 査学强; 顾东晓

doi:10.3969/j.issn.1001-5256.2014.02.018

蛋白质组学及代谢组学在酒精性肝病中的应用

DOI: 10.3969/j.issn.1001-5256.2014.02.018

1. 合肥工业大学生物与食品工程学院中草药与功能食品研究所
2. 安徽中医药大学第一附属医院药学部
3. 合肥工业大学信息管理与信息系统研究所

基金项目:

国家自然科学基金资助项目(No.31271814,No.21006019,No.71301040)；

详细信息

中图分类号: R575
计量
- 文章访问数: 3014
- HTML全文浏览量: 18
- PDF下载量: 783
- 被引次数: 0
出版历程
- 出版日期: 2014-02-20

Application of proteomics and metabonomics in studies on alcoholic liver disease

Research funding:

摘要

摘要:
酒精性肝病(ALD)是当今全球范围内倍受关注的公共卫生问题之一,其发病机制尚未完全阐明。蛋白质组学和代谢组学为ALD研究开辟了新的途径,且已取得一定进展。全面介绍了蛋白质组学和代谢组学在ALD研究中近10 a的应用现状、存在问题并提出联合蛋白质组学和代谢组学技术应用于ALD研究是一个很好的策略,不仅有助于"全景式"揭示其发病机制,还能为ALD的早期检测、临床诊断、治疗以及预后评估提供重要信息。
- 蛋白质组学 /
- 代谢 /
- 肝疾病,酒精性 /
- 综述
Abstract:
Alcoholic liver disease ( ALD) has become one of the major public health problems in the world, and its pathogenesis has not yet been completely clarified. Proteomics and metabonomics provide a new way for the studies on ALD, and some progress has been made in this regard. Here, the applications of proteomics and metabonomics in studies on ALD in recent 10 years, as well as existing problems, are comprehensively reviewed, and it is proposed that joint use of proteomics and metabonomics in studies on ALD is a good strategy, which helps to reveal the pathogenesis of ALD in a panoramic view and provide important information for the early detection, clinical diagnosis, treatment, and prognostic evaluation of ALD.
- proteomics /
- metabolism /
- liver diseases, alcoholic /
- review

HTML全文

参考文献(54)

[1]BRANDON-WARNER E, SCHRUM LW, SCHMIDT CM, et al.Rodent models of alcoholic liver disease:of mice and men[J].Alcohol, 2012, 46 (8) :715-725.

[2]DIEHI AM.Liver disease in alcohol abusers:clinical perspective[J].Alcohol, 2002, 27 (1) :7-11.

[3]O'SHEA RS, DASARATHY S, McCULLOUGH AJ.Alcoholic liver disease[J].Hepatology, 2010, 51 (1) :307-328.

[4]European Association for the Study of Liver.EASL clinical practical guidelines:management of alcoholic liver disease[J].J Hepatol, 2012, 57 (2) :399-420.

[5]JIA QY.Current treatment of alcoholic liver disease[J].Jilin J Tradit Chin Med, 2012, 32 (5) :536-537. (in Chinese) 贾秋颖.酒精性肝病治疗现状[J].吉林中医药, 2012, 32 (5) :536-537.

[6]WOO GA, O'BRIEN C.Long-term management of alcoholic liver disease[J].Clin Liver Dis, 2012, 16 (4) :763-781.

[7]BEIER JI, ARTEEL GE, McCLAIN CJ.Advances in alcoholic liver disease[J].Curr Gastroenterol Rep, 2011, 13 (1) :56-64.

[8]JEONG WI, GAO B.Innate immunity and alcoholic liver fibrosis[J].J Gastroenterol Hepatol, 2008, 23 (Suppl 1) :s112-s118.

[9]World Health Organization.Global status report on alcohol and health[M].Geneva:WHO Press, 2011.

[10]SCHWARTZ JM, REINUS JF.Prevalence and natural history of alcoholic liver disease[J].Clin Liver Dis, 2012, 16 (4) :659-666.

[11]QIAO CP, CHEN YX.Advances in study of the relationship between apoptosis and alcoholic liver disease[J].J Clin Hepatol, 2012, 28 (5) :386-390. (in Chinese) 乔春萍, 陈岳祥.细胞凋亡与酒精性肝病发病相关性的研究进展[J].临床肝胆病杂志, 2012, 28 (5) :386-390.

[12]MANDREKAR P, SZABO G.Signalling pathways in alcohol-induced liver inflammation[J].J Hepatol, 2009, 50 (6) :1258-1266.

[13]CRAWFORD JM.Histologic findings in alcoholic liver disease[J].Clin Liver Dis, 2012, 16 (4) :699-716.

[14]ZHANG LJ, JIA XF, TENG ZL, et al.Biomarkers of liver cancer related to hepatitis B virus selected by proteomics[J].J Clin Hepatol, 2011, 27 (5) :553-557. (in Chinese) 张丽军, 贾小芳, 滕珍林, 等.基于蛋白质组学筛选获得的乙型肝炎病毒相关肝癌诊断标志物的研究进展[J].临床肝胆病杂志, 2011, 27 (5) :553-557.

[15]SCHFER A, von TOERNE C, BECKER S, et al.Two-dimensional Peptide separation improving sensitivity of selected reaction monitoring-based quantitative proteomics in mouse liver tissue:comparing off-gel electrophoresis and strong cation exchange chromatography[J].Anal Chem, 2012, 84 (20) :8853-8862.

[16]WASINGER VC, CORDWELL SJ, CERPA-POLJAK A, et al.Progress with gene-product mapping of the Mollicutes:Mycoplasma genitalium[J].Electrophoresis, 1995, 16 (7) :1090-1094.

[17]WILKINS MR, APPEL RD, WILLIAMS KL, et al.Proteome Research:Concept, Technology, Application[M].Second Edition.Berlin:Springer-Verlag, 2007.

[18]FERNANDO H, WIKTOROWICZ JE, SOMAN KV, et al.Liver proteomics in progressive alcoholic steatosis[J].Toxicol Appl Pharmacol, 2013, 266 (3) :470-480.

[19]AROOR AR, LOWERY RJ, RESTREPO RJ, et al.A Proteomic analysis of liver after ethanol binge in chronically ethanol treated rats[J].Proteome Sci, 2012, 10 (1) :29.

[20]BANERJEE A, RUSSELL WK, JAYARAMAN A, et al.Identification of proteins to predict the molecular basis for the observed gender susceptibility in a rat model of alcoholic steatohepatitis by 2-D gel proteomics[J].Proteomics, 2008, 8 (20) :4327-4337.

[21]KLOUCKOVA I, HRNCIROVA P, MECHREF Y, et al.Changes in liver protein abundance in inbred alcohol-preferring rats due to chronic alcohol exposure, as measured through a proteomics approach[J].Proteomics, 2006, 6 (10) :3060-3074.

[22]KHARBANDA KK, VIGNESWARA V, Mc VICKER BL, et al.Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed rats[J].Biochem Biophys Res Commun, 2009, 381 (4) :523-527.

[23]KIM BJ, HOOD BL, ARAGON RA, et al.Increased oxidation and degradation of cytosolic proteins in alcohol-exposed mouse liver and hepatoma cells[J].Proteomics, 2006, 6 (4) :1250-1260.

[24]PARK B, OH SH, SEONG JK, et al.A strain-specific alteration of proteomic expression in mouse liver fructose 1, 6-bisphosphatase isoforms by alcohol[J].Proteomics, 2004, 4 (11) :3413-3421.

[25]YAMADA M, SATOH M, SEIMIYA M, et al.Combined Proteomic Analysis of Liver Tissue and Serum in Chronically Alcohol-Fed Rats[J].Alcohol Clin Exp Res, 2013, 37 (Suppl 1) :e79-e87.

[26]SOGAWA K, KODERA Y, SATOH M, et al.Increased serum levels of pigment epithelium-derived factor by excessive alcohol consumption-detection and identification by a three-step serum proteome analysis[J].Alcohol Clin Exp Res, 2011, 35 (2) :211-217.

[27]FREEMAN WM, SALZBERG AC, GONZALES SW, et al.Classification of alcohol abuse by plasma protein biomarkers[J].Biol Psychiatry, 2010, 68 (3) :219-222.

[28]KAUR I, KATYAL A.Immunoproteomic identification of biotransformed self-proteins from the livers of female Balb/c mice following chronic ethanol administration[J].Proteomics, 2012, 12 (12) :2036-2044.

[29]ANDRINGA KK, KING AL, ECCLESTON HB, et al.Analysis of the liver mitochondrial proteome in response to ethanol and S-adenosylmethionine treatments:novel molecular targets of disease and hepatoprotection[J].Am J Physiol Gastrointest Liver Physiol, 2010, 298 (5) :g732-g745.

[30]ZHANG L, JIA X, FENG Y, et al.Plasma membrane proteome analysis of the early effect of alcohol on liver:implications for alcoholic liver disease[J].Acta Biochim Biophys Sin (Shanghai) , 2011, 43 (1) :19-29.

[31]FRITZ KS, GALLIGAN JJ, HIRSCHEY MD, et al.Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice[J].J Proteome Res, 2012, 11 (3) :1633-1643.

[32]SHEPARD BD, TUMA DJ, TUMA PL.Chronic ethanol consumption induces global hepatic protein hyperacetylation[J].Alcohol Clin Exp Res, 2010, 34 (2) :280-291.

[33]SUH SK, HOOD BL, KIM BJ, et al.Identification of oxidized mitochondrial proteins in alcohol-exposed human hepatoma cells and mouse liver[J].Proteomics, 2004, 4 (11) :3401-3412.

[34]VENKATRAMAN A, LANDAR A, DAVIS AJ, et al.Oxidative modification of hepatic mitochondria protein thiols:effect of chronic alcohol consumption[J].Am J Physiol Gastrointest Liver Physiol, 2004, 286 (4) :g521-g527.

[35]VENKATRAMAN A, LANDAR A, DAVIS AJ, et al.Modification of the mitochondrial proteome in response to the stress of ethanoldependent hepatotoxicity[J].J Biol Chem, 2004, 279 (21) :22092-22101.

[36] NICHOLSON JK, CONNELLY J, LINDON JC, et al.Metabonomics:a platform for studying drug toxicity and gene function[J].Nat Rev Drug Discov, 2002, 1 (2) :153-161.

[37]FERNANDO H, BHOPALE KK, KONDRAGANTI S, et al.Lipidomic changes in rat liver after long-term exposure to ethanol[J].Toxicol Appl Pharmacol, 2011, 255 (2) :127-137.

[38]LIAN JS, LIU W, HAO SR, et al.A serum metabonomic study on the difference between alcohol-and HBV-induced liver cirrhosis by ultraperformance liquid chromatography coupled to mass spectrometry plus quadrupole time-of-flight mass spectrometry[J].Chin Med J:Engl, 2011, 124 (9) :1367-1373.

[39]ZIVKOVIC AM, BRUCE GERMAN J, ESFANDIARI F, et al.Quantitative lipid metabolomic changes in alcoholic micropigs with fatty liver disease[J].Alcohol Clin Exp Res, 2009, 33 (4) :751-758.

[40]LI S, LIU H, JIN Y, et al.Metabolomics study of alcohol-induced liver injury and hepatocellular carcinoma xenografts in mice[J].J Chromatogr B Analyt Technol Biomed Life Sci, 2011, 879 (24) :2369-2375.

[41]XIE G, ZHONG W, ZHENG X, et al.Chronic ethanol consumption alters Mammalian gastrointestinal content metabolites[J].J Proteome Res, 2013, 12 (7) :3297-3306.

[42]MANNA SK, PATTERSON AD, YANG Q, et al.Identification of noninvasive biomarkers for alcohol-induced liver disease using urinary metabolomics and the Ppara-null mouse[J].J Proteome Res, 2010, 9 (8) :4176-4188.

[43]MANNA SK, PATTERSON AD, YANG Q, et al.UPLC-MSbased urine metabolomics reveals indole-3-lactic acid and phenyllactic acid as conserved biomarkers for alcohol-induced liver disease in the Ppara-null mouse model[J].J Proteome Res, 2011, 10 (9) :4120-4133.

[44]QIN X, XIE X, FAN Y, et al.Peroxisome proliferator-activated receptor-delta induces insulin-induced gene-1 and suppresses hepatic lipogenesis in obese diabetic mice[J].Hepatology, 2008, 48 (2) :432-441.

[45]GOUDARZI M, KOGA T, KHOZOIE C, et al.PPARβ/δmodulates ethanol-induced hepatic effects by decreasing pyridoxal kinase activity[J].Toxicology, 2013, 311 (3) :87-98.

[46]LIEBER CS.Metabolism of alcohol[J].Clin Liver Dis, 2005, 9 (1) :1-35.

[47]SHI X, YAO D, CHEN C.Identification of N-acetyltaurine as a novel metabolite of ethanol through metabolomics-guided biochemical analysis[J].J Biol Chem, 2012, 287 (9) :6336-6349.

[48]KIM SJ, LEE JW, JUNG YS, et al.Ethanol-induced liver injury and changes in sulfur amino acid metabolomics in glutathione peroxidase and catalase double knockout mice[J].J Hepatol, 2009, 50 (6) :1184-1191.

[49]FERNANDO H, BHOPALE KK, BOOR PJ, et al.Hepatic lipid profiling of deer mice fed ethanol using1H and31P NMR spectroscopy:a dose-dependent subchronic study[J].Toxicol Appl Pharmacol, 2012, 264 (3) :361-369.

[50]HANASH S.HUPO initiatives rel evant to clinical proteomics[J].Mol Cell Proteomics, 2004, 3 (4) :298-301.

[51]ZAKHARI S, LI TK.Determinants of alcohol use and abuse:Impact of quantity and frequency patterns on liver disease[J].Hepatology, 2007, 46 (6) :2032-2039.

[52]HOOD L.Systems biology:integrating technology, biology, and computation[J].Mech Ageing Dev, 2003, 124 (1) :9-16.

[53]WESTON AD, HOOD L.Systems biology, proteomics, and the future of health care:toward predictive, preventative, and personalized medicine[J].J Proteome Res, 2004, 3 (2) :179-196.

[54]PARENT R, BERETTA L.Proteomics in the study of liver pathology[J].J Hepatol, 2005, 43 (1) :177-183.

施引文献

资源附件(0)

访问统计