慢性乙型肝炎患者血清磷脂酰肌醇-4-磷酸酶的表达与HBV DNA载量的相关性

刘昊刚; 辛永宁; 姜曼; 张丁丁; 姜相君; 宣世英

doi:10.3969/j.issn.1001-5256.2014.02.009

慢性乙型肝炎患者血清磷脂酰肌醇-4-磷酸酶的表达与HBV DNA载量的相关性

DOI: 10.3969/j.issn.1001-5256.2014.02.009

1. 青岛大学医学院
2. 青岛市市立医院消化内二科
3. 青岛市消化疾病重点实验室
4. 中国海洋大学医药学院

基金项目:

中国肝炎防治基金会天晴肝病研究基金(TQGB2011007)；青岛市科技支持计划项目(12-1-4-16-(09)-ich)；

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出版历程
- 出版日期: 2014-02-20

Correlation between expression of PI4KA in serum and HVB DNA load in patients with chronic hepatitis B

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摘要

摘要:
目的探讨慢性乙型肝炎患者血清磷脂酰肌醇-4-磷酸酶（PI4KA）表达与HBV载量的关系。方法收集2012年6月-2013年4月在青岛市立医院就诊的180例慢性乙型肝炎患者的血清,利用荧光定量PCR检测其HBV DNA的载量,根据其HBV DNA载量的高低,分为3组,每组60例:高病毒载量组（HBV DNA>1×107拷贝/ml）、中病毒载量组（1×105拷贝/ml≤HBV DNA≤1×107拷贝/ml）及低病毒载量组（HBV DNA<1×105拷贝/ml）,同时以60名健康人作对照。采用ELISA法,检测各组外周血清中的PI4KA的浓度。多组间血清HBV DNA定量的比较采用单因素方差分析,进一步两两比较采用LSD-t检验。PI4KA水平与HBV DNA载量之间的相关性分析采用Spearman检验。结果健康人血清PI4KA的浓度为（2.29±0.75）ng/ml;低、中、高病毒载量组血清PI4KA的浓度分别为（2.73±0.71）、（3.52±0.78）及（4.72±0.77）ng/ml。慢性乙型肝炎患者不同病毒载量组与健康对照组间血清PI4KA的浓度差异有统计学意义（F=...
- 肝炎病毒,乙型 /
- 肝炎,乙型,慢性 /
- 病毒载量 /
- 1-磷脂酰肌醇4-激酶
Abstract:
Objective To investigate the relationship between the expression of phosphatidylinositol 4- kinase III alpha ( PI4KA) in serum and HBV DNA load in patients with chronic hepatitis B ( CHB) . Methods Serum samples were collected from 180 patients with CHB, whose HBV DNA loads were determined by fluorescence quantitative PCR. According to the serum HBV DNA loads, the 180 patients were divided into three groups: high viral load group ( HBV DNA load > 107copies / ml) , medium viral load group ( 105copies / ml≤HBV DNA load≤107copies / ml) , and low viral load group ( HBV DNA load < 105copies / ml) , while the serum samples of 60 healthy people were selected as control group. Enzyme- linked immunosorbent assay was used to determine the concentration of PI4KA in peripheral serum. Results The concentrations of serum PI4KA were ( 2. 29 ± 0. 75) ng /ml in the control group, ( 2. 73 ± 0. 71) ng /ml in the low viral load group, ( 3. 52 ± 0. 78) ng / ml in the medium viral load group, and ( 4. 72 ± 0. 77) ng / ml in the high viral load group. There were significant differences in serum PI4KA concentration between the patient groups with different viral loads and control group ( F = 119. 958, P < 0. 01) , and the PI4KA levels in the three viral load groups were all significantly higher than those in the control group. The HBV DNA load and PI4KA concentration presented a positive correlation with each other ( r = 0. 758, P < 0. 01) - serum PI4KA level would be elevated with the increase in HBV DNA load. Conclusion The concentration of serum PI4KA is closely correlated with the HBV DNA load in patients with CHB, which suggests that PI4KA may play a crucial role in the HBV DNA replication.
- hepatitis B virus /
- hepatitis B, chronic /
- viral load /
- 1-phosphatidylinositol 4-kinase

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