Livin、Smac在肝癌中的表达及与凋亡、增殖的关系

李长新; 冯震博

Livin、Smac在肝癌中的表达及与凋亡、增殖的关系

1. 北京市顺义区中医医院病理科
2. 广西医科大学基础医学院病理学教研室

详细信息

中图分类号: R735.7
计量
- 文章访问数: 3272
- HTML全文浏览量: 17
- PDF下载量: 709
- 被引次数: 0
出版历程
- 出版日期: 2010-05-20

Expression of livin and smac and their relationship with cell apoptosis and proliferation in heptocarcinoma

摘要

摘要: 目的探讨肝癌中Livin、Smac蛋白的表达及其与细胞凋亡、增殖的关系。方法应用免疫组化二步法检测肝癌及相应癌旁肝硬化组织各41例、肝血管瘤旁正常对照组织9例中Livin、Smac及PCNA的表达;应用TUNEL法检测肝细胞的凋亡。结果 41例肝癌中Livin、Smac的阳性率分别46.34%、34.14%,相应癌旁肝硬化组织中分别为24.39%、56.10%,正常对照组织中分别为0%、77.8%,肝癌中Livin、Smac的阳性率与非癌肝组织相比差异均有统计学意义（P<0.05）;肝癌中Livin的表达与肝癌的转移与否有相关性（P<0.05）,二者与肿瘤细胞的凋亡增殖比均有相关性（P<0.05）;Livin和Smac在肝癌中的表达呈负相关（rs=-0.360,P<0.05）。结论 Livin的相对高表达,Smac的相对低表达或表达缺失在一定程度上打破了肝癌中细胞凋亡与增殖的动态平衡,在肝癌的形成过程中起重要的作用。
- 肿瘤抑制蛋白类 /
- 肝肿瘤 /
- Livin蛋白 /
- Smac蛋白
Abstract: Objective To investigate the expression of Livin and Smac proteins in hepatocarcinoma and their relationships with cell apoptosis and proliferation.Methods The expression of Livin, Smac and PCNA were assessed in 41 hepatocarcinomas, 41 adjacent cirrhosis tissues and 9 normal tissues in hemangioma by immunohistochemistry and apoptosis was detected by TUNEL method.Results The expression rate of Livin and Smac proteins were 46.34% and 34.14% in hepatocarcinoma patients, 24.39% and 56.10% in the adjacent cirrhosis tissues, and 0 and 77.8% in normal tissues hemangioma, respectively.A statistically significant correlation of Livin and Smac proteins expression was found between hepatocarcinoma and non hepatocarcinoma groups (P < 0.05) .Livin expression in hepatocarcinoma was significantly correlated with tumor metastasis (P < 0.05) , and both proteins in cancer tissues were significantly correlated with the ratio of apoptic index to proliferative index (P < 0.05) .The expression levels of Livin and Smac showed a negative correlation in hepatocarcinoma (rs =-0.360, P =0.021, P <0.05) .Conclusion The relatively high expression of Livin and the low expression or depletion of Smac proteins may alter the dynamic balance between the apoptosis and proliferation of hepatocarcinoma cells to a certain extent, and then play an important role in the oncogenesis of hepatocarcinoma.
- tumor suppressor protein /
- liver neoplasms /
- Livin /
- Smac

HTML全文

参考文献(8)

[1]Ma L, Huang Y, Song Z, et al.Livin promotes Smac/DIABLO deg-radation by ubiquitin-proteasome pathway[J].Cell Death Differ, 2006, 13 (12) ∶2079-2088.

[2]胡慧, 王从俊, 龚龙, 等.凋亡抑制因子livin在肝癌组织中的表达及其意义[J].中华肝胆外科杂志, 2007, 13 (5) ∶348.

[3]Bao ST, Gui SQ, Lin MS.Relationship between expression of Smacand Survivin and apoptosis of primary hepatocellular carcinoma[J].Hepatobiliary Pancreat Dis Int, 2006, 5 (4) ∶580-583.

[4]Stucki JW, Simon HU.Mathematical modeling of the regulation ofcaspase-3 activation and degradation[J].J Theor Biol, 2005, 234 (1) ∶123-131.

[5]Wilkinson JC, Wilkinson AS, Scott FL, et al.Neutralization ofSmac/DIABLO by Inhibitors of Apoptosis (IAPs) [J].J Bio Chem, 2004, 279 (49) ∶51082-51090.

[6]Vucic D, Franklin MC, Wallweber HJA, et al.Engineering ML-IAP to produce an extraordinarily potent caspase9 inhibitor:implica-tions for Smac-dependent anti-apoptotic activity of ML-IAP[J].Biochem J, 2005, 385 (1) ∶11-20.

[7]Eckelman BP, Salvesen GS.The human antiapoptotic proteins cIAP1and cIAP2 bind but not inhibit caspases[J].J Bio Chem, 2006, 281 (6) ∶3254-3260.

[8]Yang QH, Du CY.Smac/DIABLO selectively reduces the levels of c-IAP1 and c-IAP2 but not that of XIAP and livin in HeLacells[J].J Biol Chem, 2004, 279 (17) ∶16963-16970.

施引文献

资源附件(0)

访问统计