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The expression and significance of p16 and cyclin D1 in gallbladder carcinoma
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摘要: 目的探讨多肿瘤抑制蛋白（p16）和细胞周期蛋白（cyclin D1）的表达在胆囊癌发生发展中的作用及其对预后的影响,研究应用组织芯片在大规模高效检测临床组织样本的可行性。方法取40例胆囊癌、24例胆囊良性病变和16例癌旁黏膜标本,采用组织芯片技术制作成组织芯片,同时用S-P免疫组织化学方法检测组织芯片中p16和cyclinD1的表达。结果p16和cyclin D1在胆囊癌中的阳性表达率分别为40.0(和57.5(,与胆囊良性病变(75.0(,29.2（）和癌旁黏膜(93.8(,12.5（）相比有显著性差异（P<0.005）。p16的低表达和cyclin D1的高表达与胆囊癌的分化程度(P（0.025,P<0.01）、浸润深度（P<0.01,P<0.025）、淋巴结转移（P<0.01,P<0.01）以及患者的5年生存率（P<0.025,P<0.05）密切相关。p16和cyclinD1在胆囊癌中的表达呈负相关（r=-0.54）。结论p16和cyclin D1的异常表达在胆囊癌的发生、发展过程中起重要作用。p16和cyclin D1的表达水平可...Abstract: Objective To investigate the effect of p16 and cyclin D1 protein expressions and to evaluate their significance on the carcinogenesis, development and prognosis of the gallbladder carcinoma (GBC) and utilize tissue chip analysis on a large scale.Methods The tissue chip technique was done on 40 cases of GBC of which 24 cases were benign lesions and 16 cases were of cancer-adjacent tissues.The expressions of p16 and cyclin D1 in the tissue chip were detected by S-P immunohistochemical methods.Results The positivity rate of p16 and cyclin D1 expression in GBC tissues were 40.0%and 57.5%, respectively.These findings were significantly different from those in the benign lesions (75.0%, 29.25%) and cancer-adjacent (93.8%, 12.5%) tissues (P﹤0.005) .The low expression of p16 and the over-expression of cyclin D1 were also correlated closely with the differentiation degree (P﹤0.025, P﹤0.01) , invasion depth (P﹤0.01, P﹤0.025) , lymph node metastasis (P﹤0.01, P﹤0.01) and the five-year survival rate (P﹤0.025, P﹤0.05) of GBC patients.The p16 expression was negatively correlated with the cyclin D1 expression (r=-0.54) in GBC.Conclusion Abnormal expression of p16 and cyclin D1 plays an important role in the pathogenesis and development of GBC.The expression of p16 and cyclin D1 could be used as an indicator in the prognosis of GBC.It is feasible to utilize tissue chip for a rapid, economic and accurate screening of clinical tissue specimens on a large scale.
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[1]Sheer CJ.Mammalian G1cyclins[J].Cell, 1993, 73 (6) :1059-1065. [2]刘勇.肿瘤细胞增殖调控的研究进展[J].实用癌症杂志, 1977, 12 (2) :155-157. [3]刘勇.生物芯片技术及其在肿瘤研究中的应用[J].江西医学检验, 2002, 20 (6) :376-378. [4]Kononen J, Bubeneorf L, Kallioniemi A, et al.Tissue microarrays for high-throughputmoleu molecular profiling of tumor specimens[J].Nat Med, 1998, 4 (7) :844-847. [5]刘勇, 路名芝, 文进.细胞周期调控基因p21WAF1/CIP1在膀胱移行细胞癌中的表达[J].中华病理学杂志, 2000, 29 (6) :443-444. [6]刘勇, 路名芝, 文进.细胞周期调控因子p27、cyclin E和Ki-67在早期胃癌中的表达[J].中华普通外科杂志, 2002, 17 (6) :366. [7]Kamb A, Gruis NA, Weaver FJ, et al.A cell cycle regulator po-tentially involved in genesis of many types[J].Science, 1994, 264 (5157) :436-440. [8]刘勇, 路名芝, 李启明.肺癌中MTS1/p16和p53基因产物表达与细胞增殖的关系[J].临床与实验病理学杂志, 1998, 14 (2) :134-136. [9]刘勇, 李启明, 王夷黎, 等.p16, p53蛋白和增殖细胞抗原在早期胃癌中的表达[J].中华实验外科杂志, 1999, 16 (6) :573. [10]Masuda M, Hirakawa N, Nakasshima T, et al.cyclin D1overex-pression in primary hypoharyngeal carcinomas[J].Cancer, 1996, 78 (3) :390-395. [11]杨海玉, 刘勇, 路名芝.肺癌中抑癌基因p21和癌基因cyclin D1的表达[J].中华结核与呼吸杂志, 2000, 23 (11) :703 (附插页) . [12]徐晓军, 罗仑昆, 何振平, 等.细胞周期相关基因cyclin D1在胆管癌中的表达及其临床意义[J].中国普通外科杂志, 2004, 13 (2) :138-139. [13]董俊峰, 吴小鹏.原发性肝细胞癌Survivin, cyclin D1的表达及其临床意义[J].中国普通外科杂志, 2007, 10 (2) :156-159. [14]李宏涛, 袁晟, 李艳琴, 等.采用组织芯片技术检测大肠癌组织中p16和cyclin D1的表达[J].临床肿瘤学杂志, 2004, 9 (5) :456-458. [15]李宏涛, 熊永萍, 徐明亮, 等.组织芯片技术应用于检测乳腺癌组织中p16和cyclin D1的表达[J].中国普通外科杂志, 2008, 17 (5) :494-496. [16]袁松林, 汤辉焕.p16、cyclin D1在胆管癌中的表达及意义[J].医学临床研究, 2007, 24 (7) :1133-1135. -
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