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Distribution of hepatitis B virus genotype in Xuzhou and its clinical significance
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摘要: 研究徐州地区乙型肝炎病毒的基因型分布及其与肝功能损伤、病毒复制水平的关系。采用PCR微板杂交-ELISA技术进行HBVDNA基因分型,并结合其HBVDNA定量值和丙氨酸转氨酶结果,分析乙肝病毒基因型的临床意义。徐州地区乙肝病毒基因型B、C和D型分别占26.7%、44.0%和17.3%,在急性乙型肝炎、慢性乙型肝炎和肝硬化中C基因型的HBVDNA定量值显著高于B型者（t=2.45,2.53和2.36,P=0.027,0.018和0.029）。D型和与D型相关的基因型发生重型肝炎的百分率为51.7%。C基因型是徐州地区的优势基因型,并与较重的肝损伤有关,而B型与较轻的肝损伤有关,D型可能与重型肝炎有关。Abstract: To study the distribution of hepatitis B virus genotype in Xuzhou and clarify the genotype-related diffrence in the liver function and level of HBV DNA.HBV genotype was determined by PCR-Microplate sandwich hybridization-ELISA technique.Analysed the clinical significance combining with the value of HBV DNA and ALT.Among them genotype B was 26.7%, genotype C was 44.0%, genotype D was 17.3%.The HBV DNA value of genotype C was significantly higher than that of genotype B in AH, CHB, and HLC, (t=2.45, 2.53 and 2.36, P=0.027, 0.018 and 0.029) , genotype D and genotype which related to geonotybe D is 57.1% in fulminiant hepatic failure (FHF) , genotype C is the major genotype in Xuzhou and associated with the development of severe liver disease contrarying with genotype B, genotype D perhaps related with fulminiant hepatic failure.
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Key words:
- Hepatitis B virus /
- Genotype /
- HBV DNA
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