Effect of high-fat diet on establishment of a rat non-alcoholic steatohepatitis/liver fibrosis model

Sun LinLin; Shi Jun; Hao JingHua; Ren WanHua; Yan ChunYing; Zhang Jie; Lin XiaoYan; Cui Bin

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Mar. 2011

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Article Navigation > Journal of Clinical Hepatology > 2011 > 27(3): 254-257+272

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Effect of high-fat diet on establishment of a rat non-alcoholic steatohepatitis/liver fibrosis model

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Published Date: 2011-03-20

Abstract

Abstract

Objective This study was aimed to establish the rat model of nonalcoholic steatohepatitis/hepatic fibrosis induced by high-fat diet.Methods Sixty male Sprague-Dawley rats(N=60) were divided into control group(n=30) and model group(n=30) randomly.The rats of control group were fed with common forage and those of the model group were given high-fat diet.Ten rats in each group were terminated at 4,8 and 12 week respectively.The serum-markers were detected.The degrees of hepatic steatosis,inflammation and fibrosis were observed by HE staining and Masson staining.Masson staining was analyzed by image analysis system to achieve the fibrosis area quantitative analysis.Results The weight of Rats in both of these two groups increased.Compared to the control group,the liver index(liver weight/ body weight %),serum cholesterol,triglyceride and aminotransferase levels in the model group increased significantly after 8 weeks after which,the rats in model group were diagnosed with fatty liver disease.After 12 weeks high-fat diet,the pathological injury of the rats hepatic tissue became more and more severe,and resulted into liver fibrosis.Conclusion The rat model of nonalcoholic steatohepatitis/hepatic fibrosis has been established successfully by high-fat diet.
- liver cirrhosis,
- experimental,
- disease models

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References

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沈阳化工大学材料科学与工程学院沈阳 110142

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Effect of high-fat diet on establishment of a rat non-alcoholic steatohepatitis/liver fibrosis model

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Effect of high-fat diet on establishment of a rat non-alcoholic steatohepatitis/liver fibrosis model

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