Effects of bone marrow mesenchymal stem cells transplantation into rats with acute liver failure

Li ShuangXing; Shi LiJun; Wang XiuJie

Issue 6

Jun. 2010

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Effects of bone marrow mesenchymal stem cells transplantation into rats with acute liver failure

Published Date: 2010-06-20

Abstract

Abstract

Objective To determine the effects of bone marrow mesenchymal stem cells (BMSCs) transplantation into rats with acute liver failure.Methods Acute liver failure was surgically induced by 90% hepatectomy.20 rats were divided into BMSCs transplantation group (n = 10) and control group.In the BMSCs transplantation group, BMSCs were suspended in 0.3 ml saline with 2 × 106 cells and transplanted after 90% hepatectomy immediately.In the control group:0.3 ml saline was injected.The rat survival rate was defined as the percentage of live rats at any specific time point.Serum levels of ALT, AST, and Alb were determined on day 0, 1, 2, 3, and 7 after hepatectomy.BMSCs-derived Alb mRNA expression in liver was determined by RT-PCR on day 7.Results The survival rate of BMSCs group was 80%, while that of control group was 20%.BMSCs were detected in the liver after transplantation.Serum levels of ALT and AST were significantly decreased on day 1, 2 and 3 in BMSCs group compared to the control group.At the same time, serum levels of Alb were significantly increased.Conclusion BMSCs transplantation increases the survival rate of hepatectomy induced acute liver failure rats and promotes the recovery of liver functions.BMSCs transplantation could possibly be an alternative to liver transplantation for the treatment of liver failure.
- mesenchymal stem cells transplantation,
- bone marrow transplantation,
- liver failure

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[1]Fernández JA, Robles R, Marín C, et al.Fulminant hepatic failure and liver transplantation:experience of Virgen de la Arrixaca Hospital[J].Transplant Proc, 2003, 35 (5) ∶1852-1864.

[2]Farmer DG, Anselmo DM, Ghobrial RM, et al.Liver transplantation for fulminant hepatic failure:experience with more than200patients over a17-year period[J].Ann Surg, 2003, 237 (5) ∶666-675.

[3]William B, Timothy JS, Georg A, et al.Outcome after wait-listing for emergency liver transplantation in acute liver failure:A single centre experience[J].J Hepatol, 2009, 50 (2) ∶306-313.

[4]Duncan AW, Dorrell C, Grompe M.Stem Cells and Liver Regeneration[J].Gastroenterology, 2009, 137 (11) ∶466-481.

[5]史立军, 李双星, 孙博, 等.骨髓基质干细胞对肝细胞与肝纤维化细胞增殖影响的体外研究[J].中华肝脏病杂志, 2007, 15 (9) ∶675-678.

[6]徐洪雨, 王瑞峰, 李宝菊, 等.Notch-1蛋白在诱导骨髓间充质干细胞向肝细胞分化的研究[J].哈尔滨医科大学学报, 2009, 43 (2) ∶17-20.

[7]Kuo TK, Hung SP, Chuang CH, et al.Stem Cell Therapy for Liver Disease:Parameters Governing the Success of Using Bone Marrow[J].Gastroenterology, 2008, 134 (7) ∶2111-2121.

[8]Onga SY, Daia H, Leonga KW.Inducing hepatic differentiation of human mesenchymal stem cells in pellet culture[J].Biomaterials.2006, 27 (22) ∶4087-4097.

[9]Barry BP, Murphy JM.Mesenchymal stem cells:clinical applications and biological characterization[J].Int J Biochem Cell Biol, 2004, 13 (1) ∶568-584.

[10]Liu ZC, Chang TM.Transdifferentiation of bioencapsulated bone marrow cells into hepatocyte-like cells in the90%hepatectomized rat model[J].Liver Transpl, 2006, 12 (9) ∶566-572.

[11]Daia LJ, Lia HY, Guan LX, et al.The therapeutic potential of bone marrow-derived mesenchymal stem cells on hepatic cirrhosis[J].Stem Cell Research, 2009, 2 (1) ∶16-25.

[12]Cui YL, Meng MB, Tang H, et al.Recombinant human hepatocyte growth factor for liver failure[J].Contemporary Clinical Trials, 2008, 29 (5) ∶696-704.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Effects of bone marrow mesenchymal stem cells transplantation into rats with acute liver failure

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