Objective To investigate the relationship between CD4+CD25+ regulatory T cells and T lymphocytes immunity in tumor microenvironment.Methods we marked CD4+CD25+ Treg and CD4+ T cells in the slides tissues using Double-immunohistochemical, and marked CD8+ Tcells in the slides tissues using En Vision.Results The number of Treg cell in the tissues of HCC was more than that of in the tissue of peri-cancer, with significant statistical difference (P=0.000) .The ratio of CD4+T/CD8+T and CD4+ T cell infiltration was positive relation to the number of Treg cell in HCC tissues (r=-0.539, P=0.014, r=-0.545, P=0.000) ;however, CD8+T cell infiltration was no relation to the number of Treg cell in HCC tissues (r=-0.403, P=0.078) .Conclusion Treg cells is the main immunosupression cell in tumor microenvironment of HCC, Treg cells suppress proliferation of CD4+ T cell in a contact-dependent manner possibly so that tumor cells can escape immune surveillance and result in invasion and progression.Functional deletion of tumor-infiltrating Treg cells could enhance tumor-specific immunotherapy.
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