应用肝组织蛋白质组模式诊断乙型肝炎病毒相关性肝纤维化的初步研究

Objective To identify proteomic patterns in hepatic tissues for diagnosing early HBV related hepatic fibrosis.Methods Proteomic spectra were generated by two-dimensional gel eletrophoresis (2-DE) .A preliminary "training" set of spectra derived from analysis of 3 hepatic fibrosis tissue and 3 healthy liver tissues, a proteomic patterns that completely discriminated cancer from non-cancer was identified.The discovered proteomic patterns then was used to classify an independent set of 14 mask samples: 10 from hepatic fibrosis tissues, and 4 from healthy liver tissues.Results The discriminatory pattern correctly identified all hepatic fibrosis tissues and healthy liver tissues in the mask set.The result yielded a sensitivity of 100%, specificity of 100%.Conclusion Apparent proteome difference is detected between hepatic fibrosis tissues and healthy liver tissues.Further analysis on the different spots of interest may be found the pathogenesis of hepatic fibrosis or early diagnosis marker of hepatic fibrosis.