Role of podoplanin in hepatic stellate cell activation and liver fibrosis

Zhiyi WANG; Guangyue YANG; Wei ZHANG; Yaqiong PU; Xin ZHAO; Wenting MA; Xuling LIU; Liu WU; Le TAO; Cheng LIU

doi:10.12449/JCH240316

Volume 40 Issue 3

Mar. 2024

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Article Navigation > Journal of Clinical Hepatology > 2024 > 40(3): 533-538

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Role of podoplanin in hepatic stellate cell activation and liver fibrosis

DOI: 10.12449/JCH240316

Zhiyi WANG^{a, b},
Guangyue YANG^a,
Wei ZHANG^b,
Yaqiong PU^c,
Xin ZHAO^{a, b},
Wenting MA^{a, c},
Xuling LIU^{a, c},
Liu WU^{a, c},
Le TAO^{a, c
,
,
,},
Cheng LIU^{a, b
,
,
,}

a.
Laboratory of Liver Diseases, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine，Shanghai 200062，China
b.
Central Laboratory, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine，Shanghai 200062，China
c.
Department of Infectious Diseases，Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine，Shanghai 200062，China

Research funding:

Shanghai Natural Science Foundation (20ZR1450300);

National Natural Science Foundation of China (82004106);

Indenpent Innovation of Shanghai Putou District Project (ptkwws202112);

Indenpent Innovation of Shanghai Putou District Project (ptkwws202223);

Project of “100 Professionals Training Plan” of Shanghai Putuo District Central Hospital (2022-RCJC-02);

Project of “100 Professionals Training Plan” of Shanghai Putuo District Central Hospital (2022-RCJC-04);

Project of “100 Professionals Training Plan” of Shanghai Putuo District Central Hospital (2022-RCLH-01);

Putuo Xinglin Excellent Youth Training Program (ptxlyq2301)

More Information

Corresponding author: TAO Le， talent901036@163.com （ORCID： 0000-0002-3659-8134）; LIU Cheng， liucheng0082010@163.com （ORCID： 0000-0002-8741-6169）
Received Date: 2023-05-29
Accepted Date: 2023-07-17
Published Date: 2024-03-20

Abstract

Abstract

Objective To investigate the role and mechanism of podoplanin （PDPN） in hepatic stellate cell （HSC） activation and liver fibrosis. Methods Liver biopsy samples were collected from 75 patients with chronic hepatitis B who attended Department of Infectious Diseases， Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine， for the first time from September 2019 to June 2022， and RT-PCR and immunohistochemistry were used to measure the expression of PDPN in liver tissue of patients in different stages of liver fibrosis. A total of 12 male C57/BL6 mice were randomly divided into control group and model group. The mice in the model group were given intraperitoneal injection of 10% CCl₄， and those in the control group were injected with an equal volume of olive oil， for 6 weeks. HE staining and Sirius Red staining were used to observe liver histopathological changes； primary mouse liver cells were separated to measure the mRNA expression of PDPN in various types of cells； primary mouse HSCs were treated with PDPN protein， followed by treatment with the NF-‍κB inhibitor BAY11-708， to measure the expression of inflammatory factors in HSCs induced by PDPN. The independent-samples t test was used for comparison of normally distributed continuous data between two groups； a one-way analysis of variance was used for comparison between multiple groups， and the least significant difference t-test was used for further comparison between two groups. The Spearman correlation analysis was used to investigate data correlation. Results As for the liver biopsy samples， there was a relatively low mRNA expression level of PDPN in normal liver， and there was a significant increase in the mRNA expression level of PDPN in liver tissue of stage S3 or S4 fibrosis （all P<0.001）. Immunohistochemical staining showed that PDPN was mainly expressed in the fibrous septum and the hepatic sinusoid， and the PDPN-positive area in S4 liver tissue was significantly higher than that in S0 liver tissue （t=8.892， P=0.001）. In normal mice， PDPN was mainly expressed in the hepatic sinusoid， and there was a significant increase in the expression of PDPN in CCl₄ model mice （t=0.95， P<0.001）， mainly in the fibrous septum. RT-PCR showed a significant increase in the mRNA expression of PDPN in the CCl₄ model mice （t=11.25， P=0.002）. Compared with hepatocytes， HSCs， Kupffer cells， and bile duct endothelial cells， hepatic sinusoidal endothelial cells showed a significantly high expression level of PDPN （F=20.56， P<0.001）. Compared with the control group， the primary mouse HSCs treated by PDPN protein for 15 minutes showed significant increases in the mRNA expression levels of the inflammation-related factors TNFα， CCL3， CXCL1， and CXCR1 （all P<0.05）， and there were significant reductions in the levels of these indicators after treatment with BAY11-7082 （all P<0.05）. Conclusion There is an increase in the expression of PDPN mainly in hepatic sinusoidal endothelial cells during liver fibrosis， and PDPN regulates HSC activation and promotes the progression of liver fibrosis via the NF-κB signaling pathway.
- Hepatic Fibrosis,
- Hepatic Stellate Cell,
- Hepatic Sinus Endothelial Cell,
- Podoplanin

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Role of podoplanin in hepatic stellate cell activation and liver fibrosis

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