Association of genotype with cellular immunity and coagulation function in chronic hepatitis B virus infection patients with different disease spectrums in Sichuan,China

Objective To investigate the genotype distribution of chronic hepatitis B virus(HBV) infection patients with different disease spectrums in Sichuan, China, as well as the association of genotypes with cellular immunity and coagulation function.Methods A total of543 patients with chronic HBV infection who were hospitalized in Chengdu Public Health Medical Center from January 2015 to June 2017 were enrolled, among whom there were 31 chronic HBV carriers, 285 patients with chronic hepatitis B(CHB), 77 patients with severe hepatitis B, 81 patients with hepatitis B cirrhosis, and 69 patients with hepatocellular carcinoma(HCC). Blood samples were collected; gene microarray was used to determine the genotypes of HBV DNA, and T lymphocyte subsets and coagulation function were measured. An analysis of variance was used for comparison of continuous data between multiple groups, and the SNK-qtest was used for further comparison between two groups; the chi-square test was used for comparison of categorical data between groups.Results Among the 543 patients with chronic HBV infection, 400(73.66%) had type B infection, 123(22.65%) had type C infection, 10(1.84%) had mixed type B and C infection, and 10(1.84%) had mixed type C and D infection. There were no significant differences in the distribution of HBV genotypes,prothrombin time, and prothrombin activity in different disease spectrums between the patients with type B infection and those with type C infection(allP> 0. 05). According to the results of T lymphocyte subsets, in the severe hepatitis B group, the patients with type C infection had higher percentages of CD3+and CD3+CD4+T cells than those with type B infection; in the chronic hepatitis B group, the patients with type C infection had a higher percentage of CD3+CD8+T cells than those with type B infection; in the chronic HBV carrier group, severe hepatitis B group, and hepatitis B cirrhosis group, the patients with type C infection had a higher CD4+/CD8+ratio than those with type B infection; in the hepatitis B cirrhosis group, the patients with type C infection had lower percentages of CD3+, CD3+CD4+, and CD3+CD8+T cells than those with type B infection; in the HCC group, the patients with type C infection had lower percentages of CD3+, CD3+CD4+, and CD3+CD8+T cells and a lower CD4+/CD8+ratio than those with type B infection, with significant differences in the percentages of CD3+CD4+T cells(t =2.078,P= 0. 041) and CD3+CD8+T cells(t =6.672,P< 0. 001) between the patients with type C in-fection and those with type B infection in the hepatitis B cirrhosis group.Conclusion Types B and C are the main genotypes of chronicHBV infection in different disease spectrums in Sichuan, with type B more commonly seen than type C, and there is no difference in the dis-tribution of HBV genotypes between patients with different disease spectrums. Disorder of cellular immune function is observed in patientswith chronic HBV infection, with different immune disorders in different stages of infection, and the genotype of infection is not associatedwith coagulation function and cellular immune function.