Objective To analyze the relationship between hepatitis B virus ( HBV) DNA load and serum alanine aminotransferase ( ALT) level and to investigate the reason that ALT level changes in patients with HBV infection. Methods ALT level ( dependent variable) and HBV DNA load ( independent variable) were subjected to curve fitting using SPSS 16. 0 to establish a curve regression equation and analyze the curve regression equation. Results A power model was established by software analysis, with the following results: R2= 0. 107, F = 85. 887, P = 0. 000, b0= 47. 785, b1= 0. 075, b2= 0. The curve analysis showed that as the HBV DNA load increased, the ALT level kept rising, but at a decreasing rate; within a certain range, the increase in HBV DNA load led to little change in ALT level, which was infinitely close to a certain value. Conclusion ALT level is correlated with HBV DNA load, but HBV DNA may not be the most direct cause of change in ALT level.
流式细胞术检测慢病毒感染5天后的BEL-7404细胞周期,沉默组的G1期细胞比例显著高于对照组(49.9%±0.8% vs 44.0%±0.9%,t=8.96,P<0.001),G2/M期细胞比例显著低于对照组(15.9%±0.2% vs 17.9%±0.7%,t=9.13,P<0.001),S期细胞比例显著低于对照组(34.2%±0.6% vs 38.1%±0.5%,t=6.91,P<0.001)(图6)。
图
6
不同处理组BEL-7404细胞周期情况
Figure
6.
Cell cycle of BEL-7404 cells in different treatment groups
EME1对于哺乳动物细胞中 DNA 代谢的各个方面都很重要,Guo等[10]研究发现EME1与胃癌细胞AGS和MGC-803的增殖及侵袭能力密切相关,沉默EME1基因表达可通过抑制蛋白激酶B活化,降低糖原合酶激酶-3β、细胞周期蛋白D1,从而抑制癌细胞增殖和侵袭,促进细胞凋亡。基于中国广西人群的研究[11]发现EME1的Glu69Asp错义多态性与肝癌风险增加显著相关。EME1的另一外显子Ile350Thr变异会提高乳腺癌的易感性[12]。
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CHEN C, WANG KX, HE MW, et al. Silencing essential meiotic endonuclease 1 inhibits the proliferation of liver cancer cells: A study of related mechanisms[J]. J Clin Hepatol, 2024, 40(5): 982-988. DOI: 10.12449/JCH240518.
CHEN C, WANG KX, HE MW, et al. Silencing essential meiotic endonuclease 1 inhibits the proliferation of liver cancer cells: A study of related mechanisms[J]. J Clin Hepatol, 2024, 40(5): 982-988. DOI: 10.12449/JCH240518.