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Volume 41 Issue 3

Mar. 2025

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Article Contents

Article Navigation > Journal of Clinical Hepatology > 2025 > 41(3): 485-492

Effect of Go-Ichi-Ni-San complex subunit 1 on disease progression and chemotherapy resistance in hepatocellular carcinoma

DOI: 10.12449/JCH250314

1.
Department of Clinical Laboratory，Clinical Laboratory Center，Tumor Hospital Affiliated to Xinjiang Medical University，Urumqi 830000，China
2.
Department of Clinical Laboratory，The Fifth Affiliated Hospital of Xinjiang Medical University，Urumqi 830000，China
3.
Experimental Diagnostic Center，Beijing Tiantan Hospital，Capital Medical University，Beijing 100070，China
4.
Department of Clinical Laboratory，Xinjiang Uygur Autonomous Region People’s Hospital，Urumqi 830000，China

Research funding:

Natural Science Foundation of Xinjiang Uygur Autonomous Region (2022D01C245);

Xinjiang Uygur Autonomous Region Industy-University Cooperation and Collaborative Education Project (MRHT1000023042108)

More Information

Corresponding author: MA Xiumin， maxiumin1210@sohu.com （ORCID： 0000-0001-8011-7513）
Received Date: 2024-07-20
Accepted Date: 2024-10-08
Published Date: 2025-03-25

Abstract

Objective To investigate the role and mechanism of Go-Ichi-Ni-San complex subunit 1 （GINS1） in the progression of hepatocellular carcinoma （HCC） and the development of chemotherapy resistance. Methods The tumor database GEPIA2 was used to analyze the differential expression of GINS1 between HCC patients and healthy individuals， and pathological tissue samples were collected from 40 HCC patients who were admitted to The Affiliated Tumor Hospital of Xinjiang Medical University and the First Affiliated Hospital of Xinjiang Medical University from May 2017 to January 2021. Immunohistochemical staining was used to measure the difference in the expression of GINS1 between HCC tissue and corresponding adjacent tissue， and the correlation between the expression level of GINS1 and the clinical TNM stage of HCC was analyzed. Western blot was also used to measure the difference in the expression of GINS1 between HCC Huh7/Hep3B/Li-7/MHCC97H cell lines and normal human QSG7701 hepatocytes. The method of lentivirus transfection was used to establish the MHCC97H cell line with stable GINS1 knockdown and its negative control cell line. CCK-8 assay and colony formation assay were used to measure cell proliferative capacity； scratch assay was used to measure cell migration ability； Transwell assay was used to measure cell invasion ability； cells were treated with oxaliplatin to measure their sensitivity to chemotherapy drugs. Nude mice were used to establish a tumor-bearing model and observe the effect of GINS1 knockdown on the growth of HCC in vivo. Western Blot was used to measure the expression levels of the proteins associated with the Notch pathway and the JAK/STAT pathway. The cells were treated with the Notch receptor agonist Jagged-1 to analyze the association between GINS1 and the Notch/JAK/STAT pathway. The independent-samples t test was used for comparison of continuous data between two groups； a one-way analysis of variance was used for comparison between multiple groups， and the least significant difference t-test was used for further comparison between two groups. Results The expression of GINS1 was upregulated in HCC patients， HCC tissue， and HCC cell lines （all P<0.05）， and the expression level of GINS1 was positively correlated with the clinical TNM stage of HCC （r=0.822， P=0.011）. Compared with the negative control cells， the GINS1-knockdown MHCC97H cells showed significant reductions in proliferation， migration， and invasion activities （all P<0.01） and a significantly enhanced sensitivity to oxaliplatin （P<0.01）. Compared with the nude mice in the control group， GINS1 knockdown caused significant inhibition of tumor weight and volume in vivo in nude mice （all P<0.001）. Compared with the negative control cells， the GINS1-knockdown MHCC97H cells showed significant reductions in the expression levels of Notch1， Notch3， p-JAK2， and p-STAT3 （all P<0.05）， while there were no significant differences in the overall expression levels of JAK2 and STAT3 （P>0.05）. After Jagged-1 treatment， the GINS1-knockdown MHCC97H cells showed significant increases in proliferation， migration， and invasion activities and a significant reduction in sensitivity to oxaliplatin， as well as significant increases in the levels of p-JAK2 and p-STAT3 （all P<0.05）. Conclusion GINS1 is upregulated in HCC and can promote HCC progression and chemotherapy resistance through the Notch/JAK2/STAT3 pathway.
- Go-Ichi-Ni-San （GINS） Complex,
- Carcinoma， Hepatocellular,
- Drug Resistance， Neoplasm,
- Signal Transduction

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