Effect of Yiqi Yangyin Jiedu Huayu prescription on intestinal metabolites in liver cirrhosis with minimal hepatic encephalopathy： A study based on metabolomics

Tingting JIANG; Qun ZHANG; Xianbo WANG; Yuyong JIANG; Xiaoli LIU; Hao YU; Zhiyun YANG

doi:10.12449/JCH250312

Volume 41 Issue 3

Mar. 2025

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Article Navigation > Journal of Clinical Hepatology > 2025 > 41(3): 469-477

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Effect of Yiqi Yangyin Jiedu Huayu prescription on intestinal metabolites in liver cirrhosis with minimal hepatic encephalopathy： A study based on metabolomics

DOI: 10.12449/JCH250312

Department of Integrated Traditional Chinese and Western Medicine，Beijing Ditan Hospital，Capital Medical University，Laboratory for Clinical Medicine，Capital Medical University，Beijing 100015，China

Research funding:

National Natural Science Foundation of China (82104781);

Research and Cultivation Program of Beijing Municipal Hospital (PZ20241802);

Beijing Hospital Management Center “Peak” talent training program (DFL20191803);

High-level Public Health Technical Personnel Construction Project Training Program (Discipline Leader-02-16)

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Corresponding author: YANG Zhiyun， yangzhiyun2016@163.com （ORCID： 0000-0002-1522-0749）
Received Date: 2024-07-08
Accepted Date: 2024-08-12
Published Date: 2025-03-25

Abstract

Abstract

Objective To investigate the effect of Yiqi Yangyin Jiedu Huayu prescription on minimal hepatic encephalopathy in liver cirrhosis based on intestinal metabolomics. Methods A total of 11 patients with liver cirrhosis who were hospitalized in Beijing Ditan Hospital， Capital Medical University， from March to May 2024， and were diagnosed with MHE based on psychometric hepatic encephalopathy score were enrolled as subjects， and 11 healthy family members of the patients were enrolled as control group. Fecal samples were collected for metabolomics analysis from the control group and the patients with MHE before and after treatment with Yiqi Yangyin Jiedu Huayu prescription， and a population cohort study was conducted to investigate the effect of Yiqi Yangyin Jiedu Huayu prescription on intestinal metabolism of patients with MHE. The Fisher’s exact test was used for categorical data between two groups； the independent samples t-test was used for comparison of normally distributed continuous data between two groups， the paired t-test was used for comparision before and after treatment within the same group， and the Wilcoxon rank-sum test was used for comparison of non-normally distributed continuous data between two groups. Results A total of 29 differentially expressed metabolites were detected between the MHE group and the control group， mainly amino acids， organic acids， organic amines， carbohydrates， fatty acids， and vitamins， and there were 12 upregulated metabolites and 17 downregulated metabolites in the MHE group， which were mainly enriched in the metabolic pathways of ornithine， branched-chain amino acid， and aromatic amino acid. After the treatment with Yiqi Yangyin Jiedu Huayu prescription， 80 differentially expressed metabolites were detected in the patients with MHE， mainly carbohydrates， organic acids， and amino acids， and there were 56 upregulated metabolites and 24 downregulated metabolites， which were mainly enriched in the metabolic pathways of ornithine， branched-chain amino acid， and aromatic amino acid. Conclusion Yiqi Yangyin Jiedu Huayu prescription can exert a therapeutic effect on patients with MHE by regulating intestinal metabolism.
- Liver Cirrhosis,
- Hepatic Encephalopathy,
- Yiqi Yangyin Jiedu Huayu Prescription,
- Traditional Chinese Medicine Therapy,
- Metabolomics

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Effect of Yiqi Yangyin Jiedu Huayu prescription on intestinal metabolites in liver cirrhosis with minimal hepatic encephalopathy： A study based on metabolomics

DOI: 10.12449/JCH250312

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Effect of Yiqi Yangyin Jiedu Huayu prescription on intestinal metabolites in liver cirrhosis with minimal hepatic encephalopathy： A study based on metabolomics

DOI: 10.12449/JCH250312

Abstract

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通讯作者: 陈斌, bchen63@163.com

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