中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R

Expression and clinical significance of cell cycle protein-dependent kinase 1 and aurora kinase A in the serum of patients with hepatitis B virus-related hepatocellular carcinoma

DOI: 10.12449/JCH240717
Research funding:

National Natural Science Foundation of China (818615010);

Gansu Provincial Outstanding Graduate Student “Star of Innovation” Project (2023CXZX-754)

More Information
  • Corresponding author: MA Yanhua, 617747928@ qq.com (ORCID: 0009-0000-2240-6413)
  • Received Date: 2023-10-17
  • Accepted Date: 2024-01-16
  • Published Date: 2024-07-25
  •   Objective  To investigate the value of serum cell cycle protein-dependent kinase 1 (CDK1) and aurora kinase A (AURKA) in the diagnosis of patients with hepatitis B virus-related hepatocellular carcinoma (HBV-HCC).  Methods  A total of 50 HBV-HCC patients, 50 patients with hepatitis B virus-related liver cirrhosis (HBV-LC), and 50 chronic hepatitis B (CHB) patients who were hospitalized in Department of Gastroenterology, Gansu Provincial Hospital, from June 2022 to December 2023 were enrolled, and 50 healthy individuals, matched for age and sex, who received physical examination at Physical Examination Center during the same period of time were enrolled as control group. Related data were recorded for all patients, including age, sex, complications, and the results of routine blood test, liver function, and coagulation for the first time after admission. ELISA was used to measure the serum levels of CDK1 and AURKA. A one-way analysis of variance was used for comparison of normally distributed continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups; the Kruskal-Wallis H test was used for comparison of non-normally distributed continuous data between multiple groups and the least significant difference Bonferroni test was used for further comparison between two groups; the chi-square test or the Fisher’s exact test was used for comparison of categorical data between groups. The Spearman correlation analysis was used to investigate the correlation between CDK1 and AURKA, and the receiver operating characteristic (ROC) curve and the area under the ROC curve (AUC) were used to investigate the value of CDK1 and AURKA in the diagnosis of HBV-HCC.  Results  There were significant differences in liver function parameters between the HBV-HCC patients and the control group (all P<0.05); there were significant differences between the CHB group and the HBV-HCC group in albumin, Glb, direct bilirubin, aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), and alkaline phosphatase (all P<0.05); there were significant differences between the HBV-LC group and the HBV-HCC group in Glb, AST, and GGT (all P<0.05). The HBV-HCC group had significantly higher serum levels of CDK1 and AURKA than the HBV-LC group, the CHB group, and the control group (all P<0.05). There was a significant positive correlation between CDK1 and AURKA in the overall study population and the HBV-HCC patients (r=0.526 6 and 0.815 2, P<0.001). With the control group as reference, CDK1 had an AUC of 0.832 3 in the diagnosis of HBV-HCC, with a sensitivity of 92.86% and a specificity of 75%, and AURKA had an AUC of 0.886 6 in the diagnosis of HCC, with a sensitivity of 95.80% and a specificity of 74%. With the CHB group as reference, CDK1 had an AUC of 0.833 3 in the diagnosis of HBV-HCC, with a sensitivity of 93.75% and a specificity of 75%, and AURKA had an AUC of 0.972 7 in the diagnosis of HBV-HCC, with a sensitivity of 95.83% and a specificity of 91.67%. With the HBV-LC group as reference, CDK1 had an AUC of 0.608 5 in the diagnosis of HBV-HCC, with a sensitivity of 66.67% and a specificity of 54.17%, and AURKA had an AUC of 0.762 2 in the diagnosis of HBV-HCC, with a sensitivity of 95.83% and a specificity of 47.92%.  Conclusion  The serum levels of CDK1 and AURKA increase with the progression of hepatitis B-associated chronic liver disease, and significant increases in serum CDK1 and AURKA have a certain value in the diagnosis of HBV-HCC.

     

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    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 5.7 %其他: 5.7 %其他: 0.3 %其他: 0.3 %Falls Church: 0.3 %Falls Church: 0.3 %Tiruchi: 0.1 %Tiruchi: 0.1 %上海: 5.2 %上海: 5.2 %东莞: 0.1 %东莞: 0.1 %丽水: 1.2 %丽水: 1.2 %佛山: 0.3 %佛山: 0.3 %六安: 0.3 %六安: 0.3 %北京: 3.8 %北京: 3.8 %南宁: 0.1 %南宁: 0.1 %卡纳塔克: 0.3 %卡纳塔克: 0.3 %厦门: 0.1 %厦门: 0.1 %台州: 0.6 %台州: 0.6 %合肥: 0.1 %合肥: 0.1 %吉林: 0.3 %吉林: 0.3 %咸阳: 0.4 %咸阳: 0.4 %哥伦布: 0.1 %哥伦布: 0.1 %嘉兴: 0.6 %嘉兴: 0.6 %圣克拉拉: 0.1 %圣克拉拉: 0.1 %圣安东尼奥: 0.1 %圣安东尼奥: 0.1 %大连: 0.1 %大连: 0.1 %天津: 0.1 %天津: 0.1 %孟买: 0.6 %孟买: 0.6 %宁德: 0.1 %宁德: 0.1 %宁波: 0.9 %宁波: 0.9 %安康: 0.3 %安康: 0.3 %安顺: 0.1 %安顺: 0.1 %宿迁: 0.4 %宿迁: 0.4 %山景城: 0.1 %山景城: 0.1 %常德: 0.6 %常德: 0.6 %广州: 1.7 %广州: 1.7 %廊坊: 0.1 %廊坊: 0.1 %张家口: 3.5 %张家口: 3.5 %徐州: 0.6 %徐州: 0.6 %德阳: 0.3 %德阳: 0.3 %成都: 0.4 %成都: 0.4 %扬州: 0.3 %扬州: 0.3 %抚州: 0.3 %抚州: 0.3 %新乡: 0.3 %新乡: 0.3 %新余: 0.1 %新余: 0.1 %无锡: 0.1 %无锡: 0.1 %日照: 0.1 %日照: 0.1 %朝阳: 0.3 %朝阳: 0.3 %杭州: 0.9 %杭州: 0.9 %榆林: 0.4 %榆林: 0.4 %武汉: 0.1 %武汉: 0.1 %汕头: 0.3 %汕头: 0.3 %江门: 0.1 %江门: 0.1 %沈阳: 0.6 %沈阳: 0.6 %泰州: 0.1 %泰州: 0.1 %济南: 0.3 %济南: 0.3 %海得拉巴: 0.4 %海得拉巴: 0.4 %淮北: 0.1 %淮北: 0.1 %淮南: 0.1 %淮南: 0.1 %淮安: 0.1 %淮安: 0.1 %渭南: 0.1 %渭南: 0.1 %湖州: 0.1 %湖州: 0.1 %湘西: 0.1 %湘西: 0.1 %漳州: 0.7 %漳州: 0.7 %潍坊: 0.1 %潍坊: 0.1 %焦作: 0.3 %焦作: 0.3 %珠海: 0.1 %珠海: 0.1 %盐城: 0.1 %盐城: 0.1 %盘锦: 0.1 %盘锦: 0.1 %石家庄: 0.1 %石家庄: 0.1 %福州: 0.6 %福州: 0.6 %科泽科德: 0.1 %科泽科德: 0.1 %秦皇岛: 0.1 %秦皇岛: 0.1 %纽约: 0.1 %纽约: 0.1 %绍兴: 0.7 %绍兴: 0.7 %维沙卡帕特南: 0.1 %维沙卡帕特南: 0.1 %绵阳: 0.1 %绵阳: 0.1 %舟山: 0.6 %舟山: 0.6 %芒廷维尤: 22.7 %芒廷维尤: 22.7 %芜湖: 0.3 %芜湖: 0.3 %芝加哥: 2.2 %芝加哥: 2.2 %苏州: 0.6 %苏州: 0.6 %荆州: 0.3 %荆州: 0.3 %荆门: 0.1 %荆门: 0.1 %莫斯科: 0.1 %莫斯科: 0.1 %营口: 0.4 %营口: 0.4 %葫芦岛: 0.1 %葫芦岛: 0.1 %衢州: 0.4 %衢州: 0.4 %襄阳: 0.1 %襄阳: 0.1 %西宁: 7.7 %西宁: 7.7 %西安: 0.9 %西安: 0.9 %诺沃克: 0.3 %诺沃克: 0.3 %贵阳: 0.3 %贵阳: 0.3 %赫尔辛基: 0.1 %赫尔辛基: 0.1 %辽阳: 0.3 %辽阳: 0.3 %运城: 1.0 %运城: 1.0 %连云港: 1.0 %连云港: 1.0 %邵阳: 0.4 %邵阳: 0.4 %郑州: 0.4 %郑州: 0.4 %郴州: 0.7 %郴州: 0.7 %重庆: 1.9 %重庆: 1.9 %金华: 0.7 %金华: 0.7 %铁岭: 0.6 %铁岭: 0.6 %锦州: 0.1 %锦州: 0.1 %长春: 11.8 %长春: 11.8 %随州: 0.1 %随州: 0.1 %青岛: 0.4 %青岛: 0.4 %鞍山: 2.2 %鞍山: 2.2 %鹰潭: 0.1 %鹰潭: 0.1 %黄冈: 0.6 %黄冈: 0.6 %其他其他Falls ChurchTiruchi上海东莞丽水佛山六安北京南宁卡纳塔克厦门台州合肥吉林咸阳哥伦布嘉兴圣克拉拉圣安东尼奥大连天津孟买宁德宁波安康安顺宿迁山景城常德广州廊坊张家口徐州德阳成都扬州抚州新乡新余无锡日照朝阳杭州榆林武汉汕头江门沈阳泰州济南海得拉巴淮北淮南淮安渭南湖州湘西漳州潍坊焦作珠海盐城盘锦石家庄福州科泽科德秦皇岛纽约绍兴维沙卡帕特南绵阳舟山芒廷维尤芜湖芝加哥苏州荆州荆门莫斯科营口葫芦岛衢州襄阳西宁西安诺沃克贵阳赫尔辛基辽阳运城连云港邵阳郑州郴州重庆金华铁岭锦州长春随州青岛鞍山鹰潭黄冈

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