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慢性乙型肝炎及肝硬化患者血清血管生成素1、2及其比值变化与HBV DNA和ALT的相关性分析

林明华 常远 刘芳 黄雁翔 徐航飞

引用本文:
Citation:

慢性乙型肝炎及肝硬化患者血清血管生成素1、2及其比值变化与HBV DNA和ALT的相关性分析

DOI: 10.12449/JCH240609
基金项目: 

北京市属医学科研院所公益发展改革试点项目 (JING YI YAN 2021-10);

北京市工程技术研究中心 (BG0320)

伦理学声明: 本研究方案于2023年6月21日经由首都医科大学附属北京佑安医院伦理委员会审批,批号:京佑科伦字〔2023〕59号。
利益冲突声明:本文不存在任何利益冲突。
作者贡献声明:林明华负责课题设计,资料分析,撰写论文;常远、刘芳、黄雁翔负责收集标本,实验工作;徐航飞负责数据处理,统计分析。
详细信息
    通信作者:

    林明华, minghualin@ccmu.edu.cn (ORCID: 0000-0003-1220-461X)

Correlation of serum angiopoietin-1, angiopoietin-2, and angiopoietin-1/angiopoietin-2 ratio with HBV DNA and alanine aminotransferase in patients with chronic hepatitis B or liver cirrhosis

Research funding: 

Beijing Municipal Institute of Public Medical Research Development and Reform Pilot Project (JING YI YAN 2021-10);

Beijing Engineering Research Center for Precision Medicine and Transformation of Hepatitis and Liver Cancer (BG0320)

More Information
  • 摘要:   目的  分析血清血管生成素(Ang)1、2及其比值变化与慢性乙型肝炎(CHB)、肝硬化患者HBV DNA和ALT的相关性。  方法  选取2018年3月—2019年10月首都医科大学附属北京佑安医院收治的CHB患者99例,肝硬化患者59例,收集临床资料和血清标本;另选同期46例健康体检者作为对照组。所有患者均采用PCR法检测血清HBV DNA;采用酶联免疫吸附法检测血清Ang-1和Ang-2水平,比较各组血清Ang-1和Ang-2及Ang-1/Ang-2的差异。非正态分布的计量资料多组间比较采用Kruskal-Wallis H检验,进一步两两比较采用Bonferroni法;采用Spearman方法分析Ang-1、Ang-2、Ang-1/Ang-2与HBV DNA、ALT的相关性。  结果  与对照组(671.0 pg/mL)相比,CHB组(479.0 pg/mL)和肝硬化组(208.4 pg/mL)Ang-1水平显著降低(P值均<0.05);与CHB组相比,肝硬化组Ang-1水平显著降低(P<0.001)。与对照组(198.0 pg/mL)相比,CHB组(286.1 pg/mL)和肝硬化组(438.4 pg/mL)Ang-2水平显著升高(P值均<0.001);与CHB组相比,肝硬化组Ang-2水平显著升高(P<0.001)。与对照组(3.4)相比,CHB组(1.6)和肝硬化组(0.5)Ang-1/Ang-2比值显著降低(P值均<0.001);与CHB组相比,肝硬化组Ang-1/Ang-2比值显著降低(P<0.001)。Spearman分析显示,CHB组Ang-1与HBV DNA及ALT均呈负相关(r值分别为-0.400、-0.394,P值均˂0.001);Ang-2与HBV DNA及ALT均呈正相关(r值分别为0.365、0.351,P值均˂0.001);Ang-1/Ang-2与HBV DNA及ALT均呈负相关(r值分别为-0.463、-0.473,P值均˂0.001);而肝硬化组Ang-1、Ang-2及Ang-1/Ang-2均与HBV DNA和ALT无相关性(P值均˃0.05)。  结论  CHB、肝硬化患者血清Ang-1、Ang-2及Ang-1/Ang-2有显著改变,其中肝炎组Ang-1、Ang-2及Ang-1/Ang-2在一定程度上反映CHB患者肝损伤的程度。

     

  • 图  1  各组血清Ang-1、Ang-2表达水平及Ang-1/Ang-2比值

    Figure  1.  The levels of Ang-1、Ang-2 and radio of Ang-1/Ang-2 in groups

    表  1  各组血清Ang-1、Ang-2表达水平及Ang-1/Ang-2比值

    Table  1.   The levels of Ang-1、Ang-2 and radio of Ang-1/Ang-2 in groups

    组别 例数 Ang-1(pg/mL) Ang-2(pg/mL) Ang-1/Ang-2
    对照组 46 671.0(410.8~1 072.5) 198.0(149.3~263.1) 3.4(1.9~4.8)
    CHB组 99 479.0(320.9~740.0)1) 286.1(224.3~373.2)2) 1.6(0.8~2.6)2)
    肝硬化组 59 208.4(140.1~370.0)1)3) 438.4(338.0~590.5)2)3) 0.5(0.2~0.9)2)3)
    χ2 56.66 70.20 86.33
    P <0.001 <0.001 <0.001
    注:与对照组比较,1)P<0.05,2)P<0.001;与CHB组比较,3)P<0.001。
    下载: 导出CSV

    表  2  CHB组Ang-1、Ang-2及Ang-1/Ang-2比值与HBV DNA、ALT的相关性分析

    Table  2.   Correlation analysis of Ang-1, Ang-2, and Ang-1/Ang-2 ratios with HBV DNA and ALT in hepatitis group

    指标 HBV DNA(IU/mL) ALT(U/L)
    r P r P
    Ang-1 -0.400 <0.001 -0.394 <0.001
    Ang-2 0.365 <0.001 0.351 <0.001
    Ang-1/Ang-2 -0.463 <0.001 -0.473 <0.001
    下载: 导出CSV

    表  3  肝硬化组Ang-1、Ang-2及Ang-1/Ang-2比值与HBV DNA、ALT的相关性分析

    Table  3.   Correlation analysis of Ang-1, Ang-2, and Ang-1/Ang-2 ratios with HBV DNA and ALT in cirrhosis group

    指标 HBV DNA(IU/mL) ALT(U/L)
    r P r P
    Ang-1 0.204 0.121 -0.001 0.992
    Ang-2 0.032 0.812 -0.089 0.501
    Ang-1/Ang-2 0.141 0.287 0.007 0.960
    下载: 导出CSV
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  • 收稿日期:  2023-08-22
  • 录用日期:  2023-11-16
  • 出版日期:  2024-06-25
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