中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 6
Jun.  2013

Analysis of related factors for intrahepatic HBV cccDNA level in patients with chronic HBV infection

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  • Published Date: 2013-06-20
  • Objective To investigate the correlations of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) level in liver tissue with serum viral markers, HBV DNA levels, and pathological changes of liver tissue in patients with chronic HBV infection, and to provide a theoretical basis for evaluating the efficacy and determining the course of antiviral therapy.Methods Thirty patients with chronic HBV infection who were hospitalized from May 2007 to February 2008 were included in the study.The HBV cccDNA in liver tissue, total HBV DNA (HBV tDNA) in liver tissue, and serum HBV DNA were quantified by real-time quantitative polymerase chain reaction;the HBsAg and HBeAg in serum were quantified by chemiluminescence immunoassay.The correlations of HBV cccDNA level in liver tissue with HBV tDNA level in liver tissue, serum HBV DNA level, serum HBsAg and HBeAg levels, and the inflammation activity and fibrosis degree of liver tissue were analyzed;Pearson's simple correlation coefficient and Spearman's rank correlation coefficient were used for correlation analysis.Results HBV cccDNA was detected in all the 30 patients with chronic HBV infection, with a content ranging from 3.15×103 to 1.06×107 copies/mg.The HBV cccDNA level in liver tissue had a positive correlation with HBV tDNA level in liver tissue (r=0.375, P<0.05) but="" had="" no="" correlation="" with="" serum="" hbv="" dna="" level="" r="0.174," p="">0.05) .The HBV cccDNA level in liver tissue had a highly positive correlation with serum HBsAg level (r=0.562, P<0.001) but="" had="" no="" correlation="" with="" serum="" hbeag="" level="" r="0.152," p="">0.05) .The HBV cccDNA level in liver tissue was not correlated with the inflammation activity and fibrosis degree of liver tissue (r=0.082, P>0.05) .Conclusion The replication of HBV cccDNA is stable and moderate in patients with chronic HBV infection.Serum HBV DNA load cannot directly represent the HBV cccDNA level in liver tissue;serum HBsAg level can be used as an indicator of HBV cccDNA level in liver tissue.

     

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