Study on the apoptosis of rat hepatic stellate cells induced by kapamycin

Objective To investigate the effect of Rapamycin (RAPA) on proliferation and apoptosis of rHSCs-99 in vitro.Methods The rHSCs-99 was divided into four groups-Control group (A) , RAPA 50 nmol/L group (B) , RAPA 100 nmol/L group (C) , RAPA 150 nmol/L group (D) .After 72 h of Rapamycin interference in rHSCs-99, the MTT colorimetric assay was used to detect the proliferation of rHSCs-99;synthesis and secretion of collagen I were detected by ELISA;Annexin-V FICT/PI double-label immuno fluorescence with flow cytometry was used to examine the apoptosis of rHSCs-99;Wright-Giemsa staining method was used to observe the morphologic change of rHSCs-99;Immunocytochemical staining was used to observe the proteinum expression of Fas, P53 and Bcl-2.Results After the rHSCs-99 were interfered with RAPA, rHSCs-99 proliferation were inhibited;the content of type I was reduced;the number of Fas, P53 positive was increased and the Bcl-2 positive was decreased.Conclusion RAPA inhibits the proliferation of the rHSCs-99 and reduces the content of collagen I of ones, facilitating the cell apoptosis.The mechanism which RAPA promote rHSCs-99 apoptosis may be through opening the Fas/FasL apoptosis pathway, up-regulating Fas、P53 and inhibiting Bcl-2 expressing to come true.