Screening Chinese medicine components for inhibition of human hepatic stellate cell migration

Objective To screen the best component from six of components of traditional Chinese medicine which were confirmed with the anti-fibrosis function.Methods A trans-well chamber system was used to observe the changes of the human hepatic stellated cell line (LX-2) migration.We select human HSC cell line (LX-2) which induced by platelet derived growth factor-BB (PDGF-BB) to study the effect of inhibition of migration.Cells were incubated with 10-4mol/L-10-7mol/L concentration of Artesunate, Breviscapine, Salidroside, Salvianolic acid B, Tetrandrine, Astragalosides, respectively.Results Six kinds of Chinese medicine components can inhibit the PDGF-induced migration of LX-2.10-4mol/L Salvianolic acid B, 10-4mol/L Astragalosides, 10-4mol/L Tetrandrine and10-5mol/L Tetrandrine have different affectiveness on cell morphology.Based on the principles of the selection, 10-4mol/L Artesunate, 10-4mol/L Breviscapine, 10-5mol/L Salidroside, 10-5mol/L Salvianolic acid B, 10-6mol/L Tetrandrine and 10-6mol/L Astragalosides can significantly inhibit LX-2 migration.Of six of them, 10-5mol/L Salvianolic has the best effectiveness (P<0.05) .Conclusion 10-5mol/L Salidroside is the most effective drugs that inhibit the migration of LX-2.