中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 2
Feb.  2011

Role of DC-SIGN on the maturation and activation of dendritic cells in chronic hepatitis B virus infection

Research funding:

 

  • Published Date: 2011-02-20
  • Objective To investigate the role of DC-SIGN on the maturation and activation of dendritic cells (DC) in chronic HBV infection.Methods Highly mannosylated HBV obtained by treating HepG2.2.15 cells with the a-mannosidase I inhibitor kifunensine were added to the medium of DC derived from peripheral blood mononuclear cells on day 5 after culture.The expression rates of CDla, CD83, CD80, CD86 and HLA-DR of DCs were assayed by flow cytometry analysis, the stimulating capacity of DC was detected by MTT assay and the levels of IL-12 released by DCs were measured by ELISA on day 7.Results Compared with native HBV group, the expression of CDla, CD83, CD80, CD86 and HLA-DR of DC were significantly improved, levels of IL-12 were increased, and capacity of stimulating lymphocyte proliferation was enhanced in highly mannosylated HBV group.Furthermore these effects could be all blocked by DC-SIGN specific antibody.Conclusion DC-SIGN can promote the maturation and activation of DC after recognizing highly mannosylated HBV, native HBV may exploit demannosylation as a way to escape recognition by DC-SIGN and thereby induce DC dysfunction.

     

  • [1]Wang FS, Xing LH, Liu MX, et al.Dysfunction of peripheral blood dendritic cells from patients with chronic hepatitis B virus infection[J].World J Gastroenterol, 2001, 7 (4) :537-541.
    [2]Koppel EA, van Gisbergen KP, Geijtenbeek TB, et al.Distinct functions of DC-SIGN and its homologues L-SIGN (DC-SIGNR) and mSIGNR1 in pathogen recognition and immune regulation[J].Cell Microbiol, 2005, 7 (2) :157-165.
    [3]Op den Brouw ML, de Jong MA, Ludwig IS, et al.Branched oligosaccharide structures on HBV prevent interaction with both DC-SIGN and L-SIGN[J].J Viral Hepat, 2008, 15 (9) :675-683.
    [4]Treuheit MJ, Kosky AA, Brems DN.Inverse relationship of protein concentration and aggregation[J].Pharm Res, 2002, 19 (4) :511-516.
    [5]Duperrier K, Eljaafari A, Dezutter-Dambuyant C, et al.Distinct subsets of dendritic cells resembling dermal DCs can be generated in vitro from monocytes, in the presence of different serum supplements[J].J Immunol Methods, 2000, 238 (1-2) :119-131.
    [6]Van Montfort T, Nabatov AA, Geijtenbeek TB, et al.Efficient capture of antibody neutralized HIV-1 by cells expressing DC-SIGN and transfer to CD4+T lymphocytes[J].J Immunol, 2007, 178 (5) :3177-3185.
    [7]Geijtenbeek TB, Van Vliet SJ, Koppel EA, et al.Mycobacteria target DC-SIGN to suppress dendritic cell function[J].J Exp Med, 2003, 197 (1) :7-17.
    [8]Wei X, Decker JM, Wang S, et al.Antibody neutralization and escape by HIV-1[J].Nature, 2003, 422 (6929) :307-312.
    [9]Lee J, Park JS, Moon JY, et al.The influence of gly-cosylation on secretion, stability, and immunogenicity of recombinant HBV pre-S antigen synthesized in Saccharomyces cerevisiae[J].Biochem Biophys Res Commun, 2003, 303 (2) :427-432.
    [10]Liu M, Chen H, Luo F, et al.Deletion of N-glycosylation sites of hepatitis C virus envelope protein E1 enhances specific cellular and humoral immune responses[J].Vaccine, 2007, 25 (36) :6572-6580.
    [11]Brown KS, Ryder SD, Irving WL, et al.Mannan binding lectin and viral hepatitis[J].Immunol Lett, 2007, 108 (1) :34-44.
    [12]Hering KW, Karaveg K, Moremen KW, et al.A practical synthesis of kifunensine analogues as inhibitors of endoplasmic reticulum alpha-mannosidase I[J].J Org Chem, 2005, 70 (24) :9892-9904.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2731) PDF downloads(889) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return