Role of DC-SIGN on the maturation and activation of dendritic cells in chronic hepatitis B virus infection

Zou XiaoJing; Jiang XueQiang; Tian DeYing

Issue 2

Feb. 2011

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Role of DC-SIGN on the maturation and activation of dendritic cells in chronic hepatitis B virus infection

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Published Date: 2011-02-20

Abstract

Abstract

Objective To investigate the role of DC-SIGN on the maturation and activation of dendritic cells (DC) in chronic HBV infection.Methods Highly mannosylated HBV obtained by treating HepG2.2.15 cells with the a-mannosidase I inhibitor kifunensine were added to the medium of DC derived from peripheral blood mononuclear cells on day 5 after culture.The expression rates of CDla, CD83, CD80, CD86 and HLA-DR of DCs were assayed by flow cytometry analysis, the stimulating capacity of DC was detected by MTT assay and the levels of IL-12 released by DCs were measured by ELISA on day 7.Results Compared with native HBV group, the expression of CDla, CD83, CD80, CD86 and HLA-DR of DC were significantly improved, levels of IL-12 were increased, and capacity of stimulating lymphocyte proliferation was enhanced in highly mannosylated HBV group.Furthermore these effects could be all blocked by DC-SIGN specific antibody.Conclusion DC-SIGN can promote the maturation and activation of DC after recognizing highly mannosylated HBV, native HBV may exploit demannosylation as a way to escape recognition by DC-SIGN and thereby induce DC dysfunction.
- hepatitis B,
- chronic,
- hepatitis B virus

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References

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Role of DC-SIGN on the maturation and activation of dendritic cells in chronic hepatitis B virus infection

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Role of DC-SIGN on the maturation and activation of dendritic cells in chronic hepatitis B virus infection

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