The relationship between HBV gene mutation in HBV infected family and HLA class Ⅱ alleles

Objective To study the relationship between the characteristics of mutation in hepatitis B virus (HBV) infected family and HLA class II molecular genetic polymorphism.Methods The mutations of HBV pre-Core area 1896 and the basal core promoter (BCP) 1762/1764 were amplified and sequenced.The characteristics and frequencies of the two site mutations of HBV were analyzed in 121cases of infected family (experimental group) and 83 cases with chronic hepatitis B (control group) of non-infected family.The HLA-DQA1 and DQB1 alleles were tested using PCR/SSP.The relationship between the HLA-II polymorphism in (HBV) infected family and HBV gene mutation was analyzed.Results The variations of pre-Core area 1896, and BCP area 1762/1764 in experimental group were higher than the control group.Combination mutations in HLA-DQA1*0501and DQB1*0301 alleles, and HBV pre-Core area 1896 and BCP1762/1764 were found in the experimental group.In the mothers of HBV infected families as the first generation, the mutations in HBV pre-Core area 1896 and BCP1762/1764 of the offspring has no relationship.Conclusion The pre-Core area 1896 and BCP area 1762/1764 mutations of HBV combined with HLA-DQA1*0501 and DQB1*0301 frequencies in family infection cases were significantly higher than those patients without family infection.There may be a relationship between the pre-Core area 1896 and BCP area 1762/1764 mutations and the immune status