Molecular mechanism of apoptosis in rat hepatic stellate cells HSC-T6 induced by TRAIL

Gao Jie; Zhao YongHua; Kang Peng; Liang Ming; Li ShuChen

Issue 3

Mar. 2010

Turn off MathJax

Article Contents

Abstract

References

Article Navigation > Journal of Clinical Hepatology > 2010 > 26(3): 300-303

PDF( 1079 KB)

Molecular mechanism of apoptosis in rat hepatic stellate cells HSC-T6 induced by TRAIL

Research funding:

Published Date: 2010-03-20

Abstract

Abstract

Objective To investigate the effect and mechanism of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) on apoptosis in cultured rat hepatic stellate cells (HSCs) HSC-T6. Methods The gene expressions of α-SMA and DR5 in HSC-T6cells were measured by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) .The proliferation and apoptosis of HSC-T6 cells treated with TRAIL were detected by 3- (4, 5-dimethytthiazole-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. The Bax, Caspase3 in the HSC-T6 cell s homogenates and mitochondrion were detected by western blot. Results The gene expressions of α-SMA and DR5 in cultured rat HSC-T6cells was low in the 2nd and 4th days, increased significantly in the 8th and 12th days (P<0.05) . The proliferation of HSC-T6 cells was reduced by TRAIL.TRAIL also induced apoptosis of HSC-T6 cell s dramatically than control (P<0.05) .The mitochondrion bax and cleaved active subunits of caspase-3 were highly expressed in treated HSC-T6 cells than in the control. Conclusion TRAIL can induce apoptosis of HSC-T6 cells effectively and the high expression of DR5 and Bax may play an important role in the apoptosis.
- tumor necrosis factor-alpha,
- apoptosis,
- astrocytes,
- mitochondria

FullText(HTML)

References(9)

References

[1]蒋业贵, 李兆申.肝星状细胞与肝纤维化的研究进展[J].世界华人消化志, 2003, 11 (9) ∶1443-1446.

[2]Hague A, Hicks DJ, Hasan F, et al.Increased sensitivity to TRAIL-induced apoptosisoccurs during the adenoma to carcinoma transi-tion of colorectal carcinogenesis[J].Br J Cancer, 2005, 92 (4) ∶736-742.

[3]Taimr P, Higuchi H, Kocova E, et al.Activated stellate cells express the TRAIL receptor-2/death receptor-5and undergo TRAIL medi-ated apoptosis[J].Hepatology, 2003, 37 (1) ∶87-95.

[4]付德才, 杨世忠, 孙钰玮, 等.甲珠对肝纤维化大鼠α-SMA和TGF-β1表达的影响[J].世界华人消化杂志, 2008, 16 (3) ∶253-258.

[5]甄国华, 张珍祥, 徐永健.氯血红素对大鼠缺氧性肺动脉高压的作用[J].中华结核和呼吸感染, 2000, 23 (9) ∶569-570.

[6]Li S, Zhao Y, He X, et al.Relief of extrinsic pathway inhibition by the Bid-dependent mitochondrial release of Smac in Fas-mediated hepatocyte apoptosis[J].J Biol Chem, 2002, 277 (30) ∶26912-26920.

[7]Saile B, Knittel T, Matthes N, et al.CD95/CD95L-mediated ap-optosis of the hepatic stellate cell.A mechanism terminating uncon-trolled hepatic stellate cell proliferation during hepatic tissue repair[J].Am J Pathol, 1997, 151 (5) ∶1265-1272.

[8]Novo E, Marra F, Zamara E.Overexpression of bcl-2by activated human hepatic stellate cells:resistance to apoptosis as a mechanism of progressive hepaticfibrogenesis in humans[J].Gut, 2006, 55 (8) ∶1174-1182.

[9]白莉, 曹传平, 张映辉, 等.促凋亡蛋白Bid诱导肝细胞凋亡的机制[J].中国生物化学与分子生物学报, 2004, 20 (5) ∶670-674.

Relative Articles

[1]	Lingling ZHU, Yani ZHANG, Tingting SHI, Yang WU, Chun GAO, Xiaohui YU, Yujing HE, Jiucong ZHANG. The role of tumor necrosis factor-α in the development and progression of hepatocellular carcinoma[J]. Journal of Clinical Hepatology, 2024, 40(11): 2320-2325. doi: 10.12449/JCH241129
[2]	Jianan ZHAO, Jinghua PENG, Ping LIU, Yiyang HU. Effect of Cordyceps polysaccharides on hepatocyte apoptosis induced by tumor necrosis factor-α[J]. Journal of Clinical Hepatology, 2021, 37(6): 1368-1372. doi: 10.3969/j.issn.1001-5256.2021.06.029
[3]	Wei HaiLiang, Si MingMing, Guo Hui, Li JingTao, Li Qian, Yan ShuGuang, Ju Di. Association between mitochondria and hepatocellular injury[J]. Journal of Clinical Hepatology, 2020, 36(3): 687-689. doi: 10.3969/j.issn.1001-5256.2020.03.048
[4]	Gong Jing, Jie XinKe. Effect of endoplasmic reticulum stress and autophagy on hepatocyte apoptosis[J]. Journal of Clinical Hepatology, 2019, 35(12): 2828-2832. doi: 10.3969/j.issn.1001-5256.2019.12.041
[5]	Gao Si, Zhao XiangXuan, Lu ZaiMing. Role of cell apoptosis regulated by P53 in treatment of hepatocellular carcinoma[J]. Journal of Clinical Hepatology, 2017, 33(7): 1373-1376. doi: 10.3969/j.issn.1001-5256.2017.07.038
[6]	Pan LiuLan, Jia ShengNan, Ma JingTing, Tai JingHua, Jin ZhenJing. Effect of decoy receptor 3 gene on hepatocyte apoptosis[J]. Journal of Clinical Hepatology, 2016, 32(7): 1330-1333. doi: 10.3969/j.issn.1001-5256.2016.07.023
[7]	Chi ZhaoChun, Geng ZhangXin. Application of tumor necrosis factor antagonists in hepatic disease treatment[J]. Journal of Clinical Hepatology, 2015, 31(7): 1176-1179. doi: 10.3969/j.issn.1001-5256.2015.07.045
[8]	Zhang QiuNan, Xiao Ying, Miao RuiXin, Zhang YaQing, Zhu Ying. Changes in intestinal flora in nonalcoholic fatty liver disease: their correlation with insulin resistance index,tumor necrosis factor- α,and interleukin- 6[J]. Journal of Clinical Hepatology, 2015, 31(7): 1078-1081. doi: 10.3969/j.issn.1001-5256.2015.07.019
[9]	Pan YuanWei, Ping Jian, Xu LieMing. Research advances in main signaling pathways for inducing apoptosis of activated hepatic stellate cells[J]. Journal of Clinical Hepatology, 2014, 30(2): 182-185. doi: 10.3969/j.issn.1001-5256.2014.02.021
[10]	Wang XiXun, Jiang LiXin, Wang JingLin, Liang Jing, Zhai HuiYuan, Xia XianMing. Experimental study on hepatocyte apoptosis induced by acute biliary tract infection[J]. Journal of Clinical Hepatology, 2014, 30(11): 1164-1168. doi: 10.3969/j.issn.1001-5256.2014.11.018
[11]	Weng ShanGeng, Xu ChangGuo, Sun Ying, Shi Zheng, Lin LiJuan. Study on the apoptosis of rat hepatic stellate cells induced by kapamycin[J]. Journal of Clinical Hepatology, 2012, 28(3): 219-222.
[12]	Qiao ChunPing, Chen YueXiang. Advances in study of the relationship between apoptosis and alcoholic liver disease[J]. Journal of Clinical Hepatology, 2012, 28(5): 386-390.
[13]	Gao Jie, Xu ChunHai, Liang Ming, Yu JianWu, Li ShuChen. Effect of fibronectin on protection of rat hepatic stellate cells HSC-T6 from TRAIL-induced apoptosis[J]. Journal of Clinical Hepatology, 2011, 27(11): 1199-1202.
[14]	Cai XiaoBo, Fan JianGao, Tian LiYan, Xu ZhengJie. The effect of advanced glycation end products on biological activity of hepatic stellate cell[J]. Journal of Clinical Hepatology, 2010, 26(4): 403-406.
[15]	Zhang Biao, Sui Tao, Yu XiuYing, Cao YongXian, Yao Yuan, He Hong. Significance of detecting apoptosis of PBMC and cytokine of supernatant induced by PHA in chronic hepatitis B[J]. Journal of Clinical Hepatology, 2004, 20(3): 138-140.
[16]	Zhu YingHua, Song HongLi, Zhang Yi, Yu Guang, Han JiJin. The Clinieal study about interleukin-1 and tumor necrosis factor Alpha in hepatocirrhosis[J]. Journal of Clinical Hepatology, 2001, 17(4): 233-234.
[17]	Wang JunXue, Wang GuoJun, Cai Xiong. The effects of oxymatrine on apoptosis induced by TNF-α in human liver cell line L02[J]. Journal of Clinical Hepatology, 2001, 17(4): 210-211+217.

Supplements(0)

Cited By

Proportional views

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article Metrics

Article views (3743) PDF downloads(839)

Molecular mechanism of apoptosis in rat hepatic stellate cells HSC-T6 induced by TRAIL

Abstract

References

Relative Articles

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

Molecular mechanism of apoptosis in rat hepatic stellate cells HSC-T6 induced by TRAIL

Abstract

References

Relative Articles

Proportional views

Catalog

通讯作者: 陈斌, bchen63@163.com

Article Metrics

Proportional views

Related

About Journal

Submission Guideline

Journal Online

Hepatobiliary College

Guidelines

Export File

Citation

Format

Content