氧四环素腹腔注射制备大鼠非酒精性脂肪肝模型的实验研究

To investigate an economical and effective method to establish nonalcoholic fatty liver (NAFL) model in rats.The rats of model group were fed with fat-rich diet and injected oxytetracycline intraperitoneally.The serum ALT, AST, TG, TC, MDA, SOD and hepatic TG, TC, MDA, SOD were measured respectively at the 2nd、4th and 8th weekend inthe model group and at the 8th weekend in the control group.The liver pathological change was evaluated by light microscopy after HE staining. At the 2nd、4th and 8th weekend, the serum and hepatic ALT, AST, TG, TC, MDA in model group increased significantly, and serum and hepatic SOD decreased significantly.In model group, adipose degeneration was observed at the 2nd and the 4th weekend, and the light liver fibrosis found at the 8th weekend.The method of injecting oxytetracycline intraperitoneally and taking fat-rich food is effective and economical to establish NAFL model in rats.