Fas、FasL在慢性乙型肝炎患者肝细胞和外周血淋巴细胞上的表达

To evaluate the correlation of the expression of Fas and Fas ligand (FasL) in the hepatocytes and peripheral blood lymphocytes (PBLs) with chronic hepatitis B (CHB) to the level of HBV replication and the degree of inflammation of the liver tissues.Liver biopsy samples from 30 cases with CHB were immunohistostained for Fas and FasL by Sp method with rabbit anti-Fas monoclonal antibody.PBLs were detected by flow cytometer with mouse monoclonal antibody.At the same time, the level of HBV DNA and hepatic function of the cases were detected.Hepatitis B virus induced the expression of Fas and FasL in hepatocytes and PBLs, The expression of Fas and FasL in hepatocytes was higher with the ingrade of inflammatory activity (P<0.001) , and was negatively related to the value of albumin and alanine aminotransferase (P<0.005, P<0.001) .But it was not related to globulin or total bilirubin.Both of Fas and FasL in the PBLs were positively correlated with the level of serial HBV DNA (P<0.001) , and were not correlated with the degree of CHB (P>0.005) .The expression of Fas and FasL in hepatocytes aggravated with the grade of inflammatory activity.But they did not contribute to hepatic lesion because alanine aminotransferase did not increase.To some extent, they affected hepatocytes to synthesize albumin.The higher level HBV DNA, the higher were the expression of Fas and FasL in PBLs.This leaded lymphocytes to decrease and could not to eliminate HBV.So Fas-FasL mediated apoptosis, contributed to pathogenesis of CHB, and might play an important role on the progression of CHB.